Curcumin modulates endothelial permeability and monocyte transendothelial migration by affecting endothelial cell dynamics

Autor: Radu Tamaian, Nicolas Barber-Chamoux, Christine Morand, Dragan Milenkovic, Laurent-Emmanuel Monfoulet, Dominique Bayle, Sylvie Mercier
Přispěvatelé: Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Research and Development Department, National Institute for Research and Development for Cryogenic and Isotopic Technologies [Valcea] (ICSI), SC Biotech Corp SRL, Service de Cardiologie [CHU de Dijon], Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), COST Action FA1403-POSITIVe, Unité de Nutrition Humaine - Clermont Auvergne (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne (UCA), Service de Cardiologie
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0301 basic medicine
transendothelial migration
migration cellulaire
THP-1 Cells
[SDV]Life Sciences [q-bio]
Biochemistry
Umbilical vein
chemistry.chemical_compound
Cell Movement
curcumin
Cytoskeleton
Kinase
Chemistry
NF-kappa B
MAP Kinase Kinase Kinases
Biomechanical Phenomena
Cell biology
Molecular Docking Simulation
Endothelial stem cell
medicine.anatomical_structure
endothelial cell
Diffusion Chambers
Culture
endothelial permeability
Tumor necrosis factor alpha
Signal transduction
Rheology
Signal Transduction
monocyte adhesion
perméabilité
Protein Serine-Threonine Kinases
03 medical and health sciences
Physiology (medical)
Cell Adhesion
Human Umbilical Vein Endothelial Cells
medicine
Humans
Tumor Necrosis Factor-alpha
Gene Expression Profiling
Monocyte
Pyruvate Dehydrogenase Acetyl-Transferring Kinase
Microarray Analysis
Coculture Techniques
Cytoskeletal Proteins
curcumine
030104 developmental biology
Gene Expression Regulation
Curcumin
cell trafficking
permeability
Proto-Oncogene Proteins c-akt
cellule endotheliale
Zdroj: Free Radical Biology and Medicine
Free Radical Biology and Medicine, 2017, 112, pp.109-120. ⟨10.1016/j.freeradbiomed.2017.07.019⟩
Free Radical Biology and Medicine, Elsevier, 2017, 112, pp.109-120. ⟨10.1016/j.freeradbiomed.2017.07.019⟩
ISSN: 0891-5849
Popis: Curcumin is a phenolic compound that exhibits beneficial properties for cardiometabolic health. We previously showed that curcumin reduced the infiltration of immune cells into the vascular wall and prevented atherosclerosis development in mice. This study aimed to investigate the effect of curcumin on monocyte adhesion and transendothelial migration (TEM) and to decipher the underlying mechanisms of these actions. Human umbilical vein endothelial cells (HUVECs) were exposed to curcumin (0.5-1μM) for 3h prior to their activation by Tumor Necrosis Factor alpha (TNF-α). Endothelial permeability, monocyte adhesion and transendothelial migration assays were conducted under static condition and shear stress that mimics blood flow. We further investigated the impact of curcumin on signaling pathways and on the expression of genes using macroarrays. Pre-exposure of endothelial cells to curcumin reduced monocyte adhesion and their transendothelial migration in both static and shear stress conditions. Curcumin also prevented changes in both endothelial permeability and the area of HUVECs when induced by TNF-α. We showed that curcumin modulated the expression of 15 genes involved in the control of cytoskeleton and endothelial junction dynamic. Finally, we showed that curcumin inhibited NF-κB signaling likely through an antagonist interplay with several kinases as suggested by molecular docking analysis. Our findings demonstrate the ability of curcumin to reduce monocyte TEM through a multimodal regulation of the endothelial cell dynamics with a potential benefit on the vascular endothelial function barrier.
Databáze: OpenAIRE
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