Contrasted enzymatic cocktails reveal the importance of cellulases and hemicellulases activity ratios for the hydrolysis of cellulose in presence of xylans
| Autor: | Jean Tayeb, Caroline Rémond, Fadhel Ben Chaabane, Eve Dondelinger, Celine Cohen, Nathalie Aubry |
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| Přispěvatelé: | Fractionnement des AgroRessources et Environnement (FARE), Université de Reims Champagne-Ardenne (URCA)-Institut National de la Recherche Agronomique (INRA), IFP Energies nouvelles (IFPEN), OSEO Innovation (France), Fractionnement des AgroRessources et Environnement - UMR-A 614 (FARE), Université de Reims Champagne-Ardenne (URCA)-Institut National de la Recherche Agronomique (INRA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Remond, Caroline |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine animal structures [SDV.BIO]Life Sciences [q-bio]/Biotechnology Biophysics éthanol Cellulase Xylose 01 natural sciences Applied Microbiology and Biotechnology Hydrolysate 03 medical and health sciences chemistry.chemical_compound Hydrolysis bioraffinerie 010608 biotechnology Food science Cellulose Trichoderma reesei biology beta glucosidase Ethanol Beta-glucosidase Xylanase food and beverages β-xylosidase biology.organism_classification Xylan beta-Glucosidase beta-xylosidase Biorefinery β-Glucosidase beta xylosidase 030104 developmental biology [CHIM.POLY]Chemical Sciences/Polymers chemistry Biochemistry biology.protein Original Article |
| Zdroj: | AMB Express AMB Express, Springer Nature, 2016, 6, pp.24. ⟨10.1186/s13568-016-0196-x⟩ AMB Express (6), . (2016) |
| ISSN: | 2191-0855 |
| Popis: | International audience; Various enzymatic cocktails were produced from two Trichoderma reesei strains, a cellulase hyperproducer strain and a strain with β-glucosidase activity overexpression. By using various carbon sources (lactose, glucose, xylose, hemi-cellulosic hydrolysate) for strains growth, contrasted enzymatic activities were obtained. The enzymatic cocktails presented various levels of efficiency for the hydrolysis of cellulose Avicel into glucose, in presence of xylans, or not. These latter were also hydrolyzed with different extents according to cocktails. The most efficient cocktails (TR1 and TR3) on Avicel were richer in filter paper activity (FPU) and presented a low ratio FPU/β-glucosidase activity. Cocktails TR2 and TR5 which were produced on the higher amount of hemicellulosic hydrolysate, possess both high xylanase and β-xylosidase activities, and were the most efficient for xylans hydrolysis. When hydrolysis of Avicel was conducted in presence of xylans, a decrease of glucose release occurred for all cocktails compared to hydrolysis of Avicel alone. Mixing TR1 and TR5 cocktails with two different ratios of proteins (1/1 and 1/4) resulted in a gain of efficiency for glucose release during hydrolysis of Avicel in presence of xylans compared to TR5 alone. Our results demonstrate the importance of combining hemicellulase and cellulase activities to improve the yields of glucose release from Avicel in presence of xylans. In this context, strategies involving enzymes production with carbon sources comprising mixed C5 and C6 sugars or combining different cocktails produced on C5 or on C6 sugars are of interest for processes developed in the context of lignocellulosic biorefinery. |
| Databáze: | OpenAIRE |
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