Evidence of haptoglobin in the porcine female genital tract during oestrous cycle and its effect on in vitro embryo production

Autor: Ernesto Rodríguez-Tobón, Carla Moros-Nicolás, Francisco A. García-Vázquez, Jason W. Ross, Chiara Luongo, Rebeca López-Úbeda, Iván Hernández-Caravaca, Mª José Izquierdo-Rico, Carmen Matás, Manuel Avilés, Ascension Guillen-Martinez
Rok vydání: 2021
Předmět:
Zdroj: Scientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
Scientific Reports
ISSN: 2045-2322
DOI: 10.1038/s41598-021-90810-6
Popis: Recent evidence supports involvement of the acute phase protein haptoglobin in numerous events during mammalian reproduction. The present study represents an in-depth investigation of haptoglobin expression and secretion in the porcine oviduct and uterus, and assesses its effect on porcine in vitro embryo production. A systematic study was made of sows in different oestrous stages: late follicular, early luteal and late luteal stages. Relative haptoglobin mRNA abundance was quantified by RT-qPCR. In addition, expression of the protein was analysed by immunohistochemistry and the results were complemented by Western-blot and proteomic analyses of the oviductal and uterine fluids. In vitro porcine fertilization and embryo culture were carried out in the presence of haptoglobin. The results indicate that haptoglobin mRNA expression in the porcine oviduct and uterus is most abundant during the late luteal stage of the oestrous cycle. By means of Western blot and proteomic analyses haptoglobin presence was demonstrated in the oviduct epithelium and in the oviductal and uterine fluids in different stages of the oestrous cycle. The addition of haptoglobin during gamete co-incubation had no effect on sperm penetration, monospermy or efficiency rates; however, compared with the control group, blastocyst development was significantly improved when haptoglobin was present (haptoglobin: 64.50% vs. control: 37.83%; p
Databáze: OpenAIRE
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