Quantitation of CD62, soluble CD62, and lysosome-associated membrane proteins 1 and 2 for evaluation of the quality of stored platelet concentrates
| Autor: | K. Kannan, Minoru Fukuda, S. G. Divers, D Dempsey, R. Chervenak, K W Betzing, R F Holcombe, R M Stewart |
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| Rok vydání: | 1995 |
| Předmět: |
Blood Platelets
P-selectin Immunology Platelet Membrane Glycoproteins Biology Flow cytometry Andrology Blood cell Blood product Antigens CD medicine Immunology and Allergy Humans Secretion Platelet Microparticle Membrane Glycoproteins medicine.diagnostic_test Lysosome-Associated Membrane Glycoproteins Hematology Flow Cytometry P-Selectin medicine.anatomical_structure Biochemistry Membrane protein Solubility Evaluation Studies as Topic Lysosomes |
| Zdroj: | Transfusion. 35(4) |
| ISSN: | 0041-1132 |
| Popis: | BACKGROUND: Platelets become activated during storage, which results in secretion of granules, vesiculation of microparticles, secretion of protein, and a number of other biochemical and morphologic processes that decrease the utility of platelet concentrates stored for transfusion. STUDY DESIGN AND METHODS: To evaluate the quality of stored platelet concentrates, the cell surface expression of specific activation-dependent antigens (CD62 and lysosome-associated membrane proteins 1 and 2 [LAMP-1, LAMP-2]) on platelets stored in a hospital blood bank over a 7-day period was examined. Relative microparticle counts and the expression of CD62 by microparticles, as well as platelet concentrate supernatant levels of soluble CD62, were determined. RESULTS: The percentage of platelets expressing CD62 increased significantly from Day 1 to Day 5 (p < 0.05) of storage; the mean fluorescence values for CD62 did not. In contrast, the mean fluorescence values of LAMP-1 and LAMP-2 rose significantly (p < 0.01 and p < 0.05, respectively) between Days 1 and 5. Significant declines in CD62, LAMP-1, and LAMP-2 percent expression and mean fluorescence were seen on Day 6 of storage (p < 0.001). Microparticle numbers increased significantly during storage and correlated with levels of CD62 protein (free and membrane-bound) (r = 0.95 vs. Day 2, p < 0.05; r = 0.88 vs. Day 5, p < 0.05). CONCLUSION: Flow cytometric evaluations of the expression of cell surface CD62, LAMP-1, and LAMP-2 are complementary tests that, especially when used in conjunction with the quantitation of CD62 protein, provided a simple and effective means of evaluating the quality of platelet concentrates stored for transfusion. |
| Databáze: | OpenAIRE |
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