Impact of Carbon Nanotube Surface Chemistry on Hydrogen Oxidation by Membrane-Bound Oxygen-Tolerant Hydrogenases
| Autor: | Elisabeth Lojou, Marianne Ilbert, Oliver Lenz, Ievgen Mazurenko, Pascale Infossi, Marie-Thérèse Giudici-Orticoni, Cristina Gutierrez-Sanchez, Stefan Frielingsdorf, Karen Monsalve |
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| Přispěvatelé: | Bioénergétique et Ingénierie des Protéines (BIP ), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU) |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Hydrogenase
Inorganic chemistry 02 engineering and technology Carbon nanotube 010402 general chemistry Electrocatalyst 01 natural sciences 7. Clean energy Catalysis law.invention Electron transfer law Protein purification Electrochemistry [CHIM]Chemical Sciences ComputingMilieux_MISCELLANEOUS Aquifex aeolicus biology Chemistry 021001 nanoscience & nanotechnology biology.organism_classification 0104 chemical sciences Enzyme binding Chemical engineering 0210 nano-technology |
| Zdroj: | ChemElectroChem ChemElectroChem, 2016, ⟨10.1002/celc.201600460⟩ ChemElectroChem, Weinheim : Wiley-VCH, 2016, ⟨10.1002/celc.201600460⟩ |
| ISSN: | 2196-0216 |
| DOI: | 10.1002/celc.201600460⟩ |
| Popis: | O2-tolerant [NiFe] hydrogenases are attractive biocatalysts for utilization in H2/O2 fuel cells, thereby reducing the amount of platinum-based catalysts. The O2-tolerant membrane-bound hydrogenases isolated from Ralstonia eutropha and Aquifex aeolicus, have been previously studied at planar electrodes. The design of a powerful enzymatic fuel cell, however, requires a considerable increase in enzyme loading. Here, we immobilized the two hydrogenases on carbon nanotubes, and we demonstrate that the enzyme binding and electron transfer properties on the 3D networks rely on the same surface chemistry as with planar electrodes. We evaluate how the intrinsic properties of each hydrogenase, i.e. temperature and O2 tolerance, are affected by the immobilization on different electrode surfaces. A role of the detergent used for protein purification is especially emphasized. We also demonstrate that O2 reduction products affect more seriously the enzyme activity than molecular O2. When immobilized on pyrene-modified carbon nanotubes, both enzymes were used for the first time in a mild-temperature, membrane-less H2/O2 enzymatic fuel cell, fed with O2-rich gas mixture, opening new avenues toward the development of alternative energy supply. |
| Databáze: | OpenAIRE |
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