Thermal response of domains in cardiolipin content bilayers
| Autor: | Miquel E. Cabañas, Òscar Domènech, Jordi Hernández-Borrell, M. Teresa Montero, Antoni Morros |
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| Rok vydání: | 2007 |
| Předmět: |
Magnetic Resonance Spectroscopy
Calorimetry Differential Scanning Hexagonal crystal system Atomic force microscopy Cardiolipins Phosphatidylethanolamines Lipid Bilayers Analytical chemistry Microscopy Atomic Force Atomic and Molecular Physics and Optics Electronic Optical and Magnetic Materials Crystallography chemistry.chemical_compound Differential scanning calorimetry chemistry Suspensions Phase (matter) Content (measure theory) Thermal Mole Cardiolipin Calcium Instrumentation |
| Zdroj: | Ultramicroscopy. 107(10-11) |
| ISSN: | 0304-3991 |
| Popis: | In the study described here, supported planar bilayers (SPBs) of 1-palmitoy-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE):cardiolipin (CL) (0.8:0.2, mol/mol) were examined using atomic force microscopy (AFM). SPBs were formed from suspensions of POPE:CL (0.8:0.2, mol/mol) in inverted hexagonal (H(II)) phases (buffer containing Ca(2+)). Three laterally segregated domains which differ in height were observed at 24 degrees C. Based on the area accounted for each domain and the nominal composition of the mixture, we interpret that the higher domain is formed by CL, while the intermediate and lower domains (LDs) are formed by POPE. The three domains respond to temperature increase with relative changes in their area. At 37 degrees C, we observed that the increase in the area of the intermediate domain occurs at the expense of the LD. (31)P-nuclear magnetic resonance ((31)P-NMR) and Differential scanning calorimetry (DSC) were used in combination with AFM to characterize the phase behavior of the suspensions and to elucidate the nature of the structures observed. |
| Databáze: | OpenAIRE |
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