Screening of bacterial DNA in bile sampled from healthy dogs and dogs suffering from liver- or gallbladder-associated disease

Autor: Sakurako NEO, Iyo TAKEMURA-UCHIYAMA, Jumpei UCHIYAMA, Hironobu MURAKAMI, Ayaka SHIMA, Hideki KAYANUMA, Taiki YOKOYAMA, Satoshi TAKAGI, Eiichi KANAI, Masaharu HISASUE
Rok vydání: 2022
Předmět:
Zdroj: The Journal of veterinary medical science. 84(7)
ISSN: 1347-7439
Popis: Although the biliary system is generally aseptic, gallbladder microbiota has been reported in humans and some animals apart from dogs. We screened and analyzed the bacterial deoxyribonucleic acid in canine gallbladders using bile sampled from 7 healthy dogs and 52 dogs with liver- or gallbladder-associated disease. PCR screening detected bacteria in 17.3% of diseased dogs (9/52) and none in healthy dogs. Microbiota analysis of PCR-positive samples showed that the microbial diversity differed between liver- and gallbladder-associated disease groups. Thus, a specific bacterial community appears to occur at a certain frequency in the bile of diseased dogs.
Databáze: OpenAIRE