Inactivation of Gram-Positive Bacteria by Novel Phenolic Branched-Chain Fatty Acids
| Autor: | Helen L. Ngo, Karen Wagner, Xuetong Fan, Kimberly J. B. Sokorai |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Gram-positive bacteria 030106 microbiology Microbial Sensitivity Tests Gram-Positive Bacteria medicine.disease_cause digestive system Microbiology 03 medical and health sciences chemistry.chemical_compound 0404 agricultural biotechnology Phenols Gram-Negative Bacteria medicine skin and connective tissue diseases Escherichia coli Pseudomonas tolaasii chemistry.chemical_classification biology Fatty Acids Fatty acid 04 agricultural and veterinary sciences biology.organism_classification Antimicrobial 040401 food science digestive system diseases Anti-Bacterial Agents Oleic acid chemistry Biochemistry Bacteria Food Science Enterococcus faecium |
| Zdroj: | Journal of Food Protection. 80:6-14 |
| ISSN: | 0362-028X |
| Popis: | Novel phenolic branched-chain fatty acids (PBC-FAs) were evaluated for their antimicrobial properties against both gram-positive ( Listeria innocua , Bacillus subtilis , Enterococcus faecium ) and gram-negative ( Escherichia coli , Salmonella Typhimurium, and Pseudomonas tolaasii ) bacteria. In addition, PBC-FA derivatives, such as PBC-FA methyl ester mixture, methyl-branched fatty acid mixtures, and trimethylsilyl-PBC-FA methyl esters, were synthesized to study the structure activity relationship. Results showed that PBC-FAs were a potent antimicrobial against gram-positive bacteria with MICs of 1.8 to 3.6 μg/ml. The compounds were less effective against gram-negative bacteria. Derivatives of PBC-FAs and an equimolar mixture of oleic acid and phenol all had MICs above 233 μg/ml against both gram-positive and gram-negative bacteria. Comparison of antimicrobial activities of the PBC-FAs with those of the derivatives suggests that the carboxylic group in the fatty acid moiety and the hydroxyl group on the phenol moiety were responsible for the antimicrobial efficacy. Growth curves of L. innocua revealed that PBC-FAs prevented bacterial growth, while MBC-FAs only delayed the onset of rapid growth of L. innocua . Our results demonstrated that the novel PBC-FAs have potential for use as antimicrobials against gram-positive bacteria. |
| Databáze: | OpenAIRE |
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