CRISPR/Cas9 gene editing uncovers the roles of CONSTITUTIVE TRIPLE RESPONSE 1 and REPRESSOR OF SILENCING 1 in melon fruit ripening and epigenetic regulation
| Autor: | Andrea Giordano, Miguel Santo Domingo, Leandro Quadrana, Marta Pujol, Ana Montserrat Martín-Hernández, Jordi Garcia-Mas |
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| Přispěvatelé: | Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), European Commission, Generalitat de Catalunya, Producció Vegetal, Genòmica i Biotecnologia, Centre for Research in Agricultural Genomics (CRAG), Institut des Sciences des Plantes de Paris-Saclay (IPS2 (UMR_9213 / UMR_1403)), Université d'Évry-Val-d'Essonne (UEVE)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) |
| Rok vydání: | 2022 |
| Předmět: |
Gene Editing
Cucurbits Physiology [SDV]Life Sciences [q-bio] technology industry and agriculture food and beverages Plant Science Ethylenes Protein-Tyrosine Kinases Epigenesis Genetic Melon Cucurbitaceae Ethylene Solanum lycopersicum Gene Expression Regulation Plant Cucumis melo DNA demethylase Fruit Proto-Oncogene Proteins CRISPR Climacteric fruit ripening CRISPR-Cas Systems Plant Proteins |
| Zdroj: | IRTA Pubpro. Open Digital Archive Institut de Recerca i Tecnologia Agroalimentàries (IRTA) Dipòsit Digital de Documents de la UAB Universitat Autònoma de Barcelona Journal of Experimental Botany Journal of Experimental Botany, 2022, 73 (12), pp.4022-4033. ⟨10.1093/jxb/erac148⟩ |
| ISSN: | 1460-2431 0022-0957 |
| Popis: | Melon (Cucumis melo) has emerged as an alternative model to tomato for studying fruit ripening due to the coexistence of climacteric and non-climacteric varieties. Previous characterization of a major quantitative trait locus (QTL), ETHQV8.1, that is able to trigger climacteric ripening in a non-climacteric background resulted in the identification of a negative regulator of ripening CTR1-like (MELO3C024518) and a putative DNA demethylase ROS1 (MELO3C024516) that is the orthologue of DML2, a DNA demethylase that regulates fruit ripening in tomato. To understand the role of these genes in climacteric ripening, in this study we generated homozygous CRISPR knockout mutants of CTR1-like and ROS1 in a climacteric genetic background. The climacteric behavior was altered in both loss-of-function mutants in two growing seasons with an earlier ethylene production profile being observed compared to the climacteric wild type, suggesting a role of both genes in climacteric ripening in melon. Single-cytosine methylome analyses of the ROS1-knockout mutant revealed changes in DNA methylation in the promoter regions of the key ripening genes such as ACS1, ETR1, and ACO1, and in transcription factors associated with ripening including NAC-NOR, RIN, and CNR, suggesting the importance of ROS1-mediated DNA demethylation for triggering fruit ripening in melon. This work was supported by Grant RTI2018-097665-B-C2 funded by MCIN/AEI/ 10.13039/501100011033, and by ‘ERDF A way of making Europe’, Severo Ochoa Programme for Centres of Excellence in R&D CEX2019-000902 also funded by MCIN/AEI/ 10.13039/501100011033, and the CERCA Programme/Generalitat de Catalunya to JG-M. AG was supported by the European Union’s Horizon 2020 research and innovation programme under Marie Skłodowska-Curie (grant agreement No. 793090). MSD was supported by a FPI grant from the Spanish Ministry of Economy and Competitiveness BES-2017-079956 funded by MCIN/AEI/ 10.13039/501100011033 and by ‘ESF Investing in your future’. Work in the Quadrana group is supported by the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program (grant agreement no. 948674). With funding from the Spanish government through the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2019-000902) |
| Databáze: | OpenAIRE |
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