Intracellular lipid binding protein family diversity from Oyster Crassostrea gigas: genomic and structural features of invertebrate lipid transporters
Autor: | Jacó Joaquim Mattos, Nestor Cubas Wendt, Guilherme Toledo-Silva, Afonso Celso Dias Bainy, Flávia L. Zacchi, Guilherme Razzera |
---|---|
Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
Oyster Protein family Ligand Binding Protein 010501 environmental sciences 01 natural sciences Article 03 medical and health sciences biology.animal Gene Duplication Gene expression Gene family Animals Crassostrea 0105 earth and related environmental sciences Regulation of gene expression Multidisciplinary biology Alternative splicing Anatomy Pacific oyster biology.organism_classification Lipid Metabolism Molecular Docking Simulation Alternative Splicing 030104 developmental biology Biochemistry Multigene Family Carrier Proteins |
Zdroj: | Scientific Reports |
ISSN: | 2045-2322 |
Popis: | Intracellular lipid binding proteins (iLBPs) play a role in the transport and cellular uptake of fatty acids and gene expression regulation. The aim of this work was to characterize the iLBP gene family of the Pacific oyster Crassostrea gigas, one of the most cultivated marine bivalves in the world, using bioinformatics and molecular biology approaches. A total of 26 different iLBPs transcripts were identified in the Pacific oyster genome, including alternative splicing and gene duplication events. The oyster iLBP gene family seems to be more expanded than in other invertebrates. Furthermore, 3D structural modeling and molecular docking analysis mapped the main amino acids involved in ligand interactions, and comparisons to available protein structures from vertebrate families revealed new binding cavities. Ten different CgiLBPs were analyzed by quantitative PCR in various tissues of C. gigas, which suggested differential prevalent gene expression of CgiLBPs among tissue groups. The data indicate a wider repertoire of iLBPs in labial palps, a food-sorting tissue. The different gene transcription profiles and reported docking systems suggest that the iLBPs are a non-generalist ligand binding protein family with specific functions. |
Databáze: | OpenAIRE |
Externí odkaz: |