Differences in drug sensitivity between two-dimensional and three-dimensional culture systems in triple-negative breast cancer cell lines
| Autor: | Masako Muguruma, Masahiko Kuroda, Kana Miyahara, Miki Okazaki, Saeko Teraoka, Mariko Asaoka, Ai Ueda, Takahiko Kawate, Takashi Ishikawa, Yohei Miyagi |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Cell Survival Cell Culture Techniques Biophysics Antineoplastic Agents Triple Negative Breast Neoplasms Docetaxel Cell morphology Biochemistry Inhibitory Concentration 50 03 medical and health sciences 0302 clinical medicine Breast cancer Cell Line Tumor Spheroids Cellular medicine Humans Viability assay Molecular Biology Triple-negative breast cancer Cell Proliferation Epirubicin Cisplatin Chemistry Cell Biology medicine.disease 030104 developmental biology Drug Resistance Neoplasm Cell culture 030220 oncology & carcinogenesis Cancer research Female medicine.drug |
| Zdroj: | Biochemical and Biophysical Research Communications. 533:268-274 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2020.08.075 |
| Popis: | Three-dimensional (3D) culture reflects tumor biology complexities compared with two-dimensional (2D) culture. Thus, 3D culture has attracted attention in cell biology studies including drug sensitivity tests. Herein, we investigated differences in anticancer drug sensitivities between 2D and 3D culture systems in triple-negative breast cancer (TNBC) cell lines. Thirteen TNBC cell lines were maintained in 2D and 3D cultures for 3 days before drug exposure. Cell morphology in the 3D culture was examined by phase-contrast microscopy. Sensitivities to epirubicin (EPI), cisplatin (CDDP), and docetaxel (DTX) were investigated by cell viability assay in both cultures and compared. The IC50s of all 3 drugs were significantly higher in the 3D culture than in the 2D culture in most cell lines. Those were correlated between the 2D and 3D cultures in EPI (R = 0.555) and CDDP (R = 0.955), but not in DTX (R = 0.221). Round spheroid-forming cells were more resistant to agents than grape-like types. In conclusion, 3D culture was more resistant to all 3 drugs than 2D culture in most TNBC cell lines. Sensitivity to CDDP was highly correlated between the 2D and 3D cultures, but not to DTX. 2D culture may be acceptable for sensitivity test for DNA-damaging agents. |
| Databáze: | OpenAIRE |
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