Cryogenic 3D printing of heterogeneous scaffolds with gradient mechanical strengths and spatial delivery of osteogenic peptide/TGF-β1 for osteochondral tissue regeneration
| Autor: | Lu Bai, Xudong Lin, Min Wang, Sanbiao Liu, Bingheng Lu, Chong Wang, Haibing Yue, Wei Huang, Xiao He, Xiaoqiong Xie, Zhi He, Yen Wei |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Calcium Phosphates
Scaffold Materials science Cell Survival 0206 medical engineering Biomedical Engineering 3D printing Biocompatible Materials Bioengineering Peptide 02 engineering and technology Biochemistry Transforming Growth Factor beta1 Biomaterials Polylactic Acid-Polyglycolic Acid Copolymer medicine Animals Regeneration chemistry.chemical_classification Tissue Engineering Tissue Scaffolds business.industry Cartilage Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells General Medicine 021001 nanoscience & nanotechnology Chondrogenesis 020601 biomedical engineering Rats medicine.anatomical_structure chemistry Printing Three-Dimensional Peptides 0210 nano-technology business Layer (electronics) Biotechnology Transforming growth factor Biomedical engineering |
| Zdroj: | Biofabrication. 12:025030 |
| ISSN: | 1758-5090 |
| DOI: | 10.1088/1758-5090/ab7ab5 |
| Popis: | Due to the increasing aging population and the high probability of sport injury among young people nowadays, it is of great demand to repair/regenerate diseased/defected osteochondral tissue. Given that osteochondral tissue mainly consists of a subchondral layer and a cartilage layer which are structurally heterogeneous and mechanically distinct, developing a biomimetic bi-phasic scaffold with excellent bonding strength to regenerate osteochondral tissue is highly desirable. Three-dimensional (3D) printing is advantageous in producing scaffolds with customized shape, designed structure/composition gradients and hence can be used to produce heterogeneous scaffolds for osteochondral tissue regeneration. In this study, bi-layered osteochondral scaffolds were developed through cryogenic 3D printing, in which osteogenic peptide/β-tricalcium phosphate/poly(lactic-co-glycolic acid) water-in-oil composite emulsions were printed into hierarchically porous subchondral layer while poly(D,L-lactic acid-co-trimethylene carbonate) water-in-oil emulsions were printed into thermal-responsive cartilage frame on top of the subchondral layer. The cartilage frame was further filled/dispensed with transforming growth factor-β1 loaded collagen I hydrogel to form the cartilage module. Although the continuously constructed osteochondral scaffolds had distinct microscopic morphologies and varied mechanical properties at the subchondral zone and cartilage zone at 37 °C, respectively, the two layers were closely bonded together, showing excellent shear strength and peeling strength. Rat bone marrow derived mesenchymal stem cells (rBMSCs) exhibited high viability and proliferation at both subchondral- and cartilage layer. Moreover, gradient rBMSC osteogenic/chondrogenic differentiation was obtained in the osteochondral scaffolds. This proof-of-concept study provides a facile way to produce integrated osteochondral scaffolds for concurrently directing rBMSC osteogenic/chondrogenic differentiation at different regions. |
| Databáze: | OpenAIRE |
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