Neoangiogenesis of human mesenchymal stem cells transfected with peptide-loaded and gene-coated PLGA nanoparticles
| Autor: | Keun-Hong Park, Jae Hwan Kim, Se Won Yi, Han Na Yang, Ji Sun Park |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Materials science Angiogenesis Biophysics Neovascularization Physiologic Bioengineering macromolecular substances 02 engineering and technology Transfection Biomaterials Neovascularization Mice 03 medical and health sciences chemistry.chemical_compound Polylactic Acid-Polyglycolic Acid Copolymer medicine Animals Humans Lactic Acid Cells Cultured Mice Inbred BALB C Matrigel Polyethylenimine Mesenchymal stem cell technology industry and agriculture Mesenchymal Stem Cells 021001 nanoscience & nanotechnology Molecular biology Cell biology Vascular endothelial growth factor PLGA 030104 developmental biology chemistry Mechanics of Materials Ceramics and Composites Nanoparticles Female medicine.symptom Peptides 0210 nano-technology Polyglycolic Acid |
| Zdroj: | Biomaterials. 76:226-237 |
| ISSN: | 0142-9612 |
| DOI: | 10.1016/j.biomaterials.2015.10.062 |
| Popis: | Several factors are involved in angiogenesis. To form new blood vessels, we fabricated vehicles carrying an angiogenesis-related peptide (apelin) and gene (vascular endothelial growth factor (VEGF)165) that were internalized by human mesenchymal stem cells (hMSCs). These non-toxic poly-(DL)-lactic-co-glycolic acid (PLGA) nanoparticles (NPs) easily entered hMSCs without cytotoxicity. The negatively charged outer surface of PLGA NPs can be easily complexed with highly positively charged polyethylenimine (PEI) to deliver genes into cells. PLGA NPs complexed with PEI could be coated with negatively charged VEGF plasmid DNA and loaded with apelin. The physical characteristics of these PLGA NPs were determined by size distribution, gel retardation, and morphological analyses. Transfection of VEGF-coated apelin-loaded PLGA NPs resulted in the differentiation of hMSCs into endothelial cells and vascular formation in Matrigel in vitro. Following injection of hMSCs transfected with these PLGA NPs into an ischemic hind limb mouse model, these cells differentiated into endothelial cells and accelerated neovascularization. |
| Databáze: | OpenAIRE |
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