Ultrasensitive DNA detection based on Au nanoparticles and isothermal circular double-assisted electrochemiluminescence signal amplification

Autor:	Hong-Yuan Chen, Hong Zhou, Jing-Juan Xu, Jing Liu
Rok vydání:	2011
Předmět:	Materials science Analytical chemistry Nanoparticle Metal Nanoparticles DNA-Directed DNA Polymerase Sulfides Photochemistry Catalysis Isothermal process Endonuclease chemistry.chemical_compound Materials Chemistry Cadmium Compounds Electrochemiluminescence Polymerase biology technology industry and agriculture Metals and Alloys General Chemistry DNA Electrochemical Techniques Endonucleases Surfaces Coatings and Films Electronic Optical and Magnetic Materials Nanocrystal chemistry Luminescent Measurements Ceramics and Composites biology.protein Gold Biosensor
Zdroj:	Chemical communications (Cambridge, England). 47(29)
ISSN:	1364-548X
Popis:	We report here an ultrasensitive DNA detection approach which combines Au NPs enhanced electrochemiluminescence (ECL) of the CdS nanocrystal (NC) film with isothermal circular amplification reaction of polymerase and nicking endonuclease (NEase). By the double-signal amplification, this approach could sensitively respond down to 5 aM DNA.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::edbde911cde2292a66743dac5e3df466 https://pubmed.ncbi.nlm.nih.gov/21695310 Zobrazit plný text záznamu