Ultrasensitive Electrochemical Detection of Clostridium perfringens DNA Based Morphology-Dependent DNA Adsorption Properties of CeO2 Nanorods in Dairy Products
| Autor: | Limei Zuo, Xin Ran, Qing Qu, Lei Li, Qiang Wang, Rui Huang, Xingcan Qian |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Clostridium perfringens
02 engineering and technology medicine.disease_cause lcsh:Chemical technology 01 natural sciences Biochemistry label-free Analytical Chemistry CeO2 nanorods chemistry.chemical_compound Adsorption medicine lcsh:TP1-1185 Electrical and Electronic Engineering Instrumentation Detection limit Chemistry Oligonucleotide Hybridization probe 010401 analytical chemistry 021001 nanoscience & nanotechnology Combinatorial chemistry Atomic and Molecular Physics and Optics 0104 chemical sciences electrochemical DNA biosensor Differential pulse voltammetry 0210 nano-technology Biosensor DNA |
| Zdroj: | Sensors, Vol 18, Iss 6, p 1878 (2018) Sensors Volume 18 Issue 6 |
| ISSN: | 1424-8220 |
| Popis: | Foodborne pathogens such as Clostridium perfringens can cause diverse illnesses and seriously threaten to human health, yet far less attention has been given to detecting these pathogenic bacteria. Herein, two morphologies of nanoceria were synthesized via adjusting the concentration of NaOH, and CeO2 nanorod has been utilized as sensing material to achieve sensitive and selective detection of C. perfringens DNA sequence due to its strong adsorption ability towards DNA compared to nanoparticle. The DNA probe was tightly immobilized on CeO2/chitosan modified electrode surface via metal coordination, and the DNA surface density was 2.51 × 10&minus 10 mol/cm2. Under optimal experimental conditions, the electrochemical impedance biosensor displays favorable selectivity toward target DNA in comparison with base-mismatched and non-complementary DNA. The dynamic linear range of the proposed biosensor for detecting oligonucleotide sequence of Clostridium perfringens was from 1.0 × 14 to 1.0 × 7 mol/L. The detection limit was 7.06 × 15 mol/L. In comparison, differential pulse voltammetry (DPV) method quantified the target DNA with a detection limit of 1.95 × 15 mol/L. Moreover, the DNA biosensor could detect C. perfringens extracted DNA in dairy products and provided a potential application in food quality control. |
| Databáze: | OpenAIRE |
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