Maternal benzo[a]pyrene exposure is correlated with the meiotic arrest and quality deterioration of offspring oocytes in mice
| Autor: | Hengye Zhang, Huiting Zhang, Kehuan Lu, Kexin Cui, Lumin Sui, Ke Yan, Xing-Wei Liang, Jiahao Liu, Peng Xiao, Junyu Nie |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
animal structures
Offspring 010501 environmental sciences Biology Toxicology complex mixtures 01 natural sciences Andrology 03 medical and health sciences chemistry.chemical_compound Pregnancy Benzo(a)pyrene polycyclic compounds medicine Animals Maternal-Fetal Exchange 030304 developmental biology 0105 earth and related environmental sciences chemistry.chemical_classification Mice Inbred ICR 0303 health sciences Reactive oxygen species organic chemicals Embryo DNA Methylation medicine.disease Oocyte Mitochondria In vitro maturation Meiosis medicine.anatomical_structure chemistry Apoptosis Prenatal Exposure Delayed Effects embryonic structures Carcinogens Oocytes Female Reactive Oxygen Species Mutagens |
| Zdroj: | Reproductive Toxicology. 93:10-18 |
| ISSN: | 0890-6238 |
| Popis: | Benzo[a]pyrene (BaP) is a polycyclic aromatic hydrocarbon (PAH) in particulate matter that has a diameter of ≤2.5 μm (PM2.5). Studies have demonstrated that BaP exposure causes oocyte meiotic arrest in mice. However, whether BaP exposure also affects oocyte maturation in offspring remains unclear. To test this, female mice were administered BaP before pregnancy to generate BaP-exposed offspring. Our findings showed that BaP exposure reduced the in vitro maturation and increased the abnormalities of meiotic apparatus in offspring oocytes. In addition, BaP exposure reduced the mitochondrial content and intracellular ATP generation, induced early apoptosis, increased reactive oxidative species accumulation and the genomic DNA 5-methylcytosine (5mc) level in offspring oocytes. Along with the abovementioned defective parameters, maternal BaP exposure further compromised the embryo developmental competence of offspring oocytes. In summary, our study demonstrated that maternal BaP exposure compromised offspring oocyte maturation and quality. |
| Databáze: | OpenAIRE |
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