Jujube honey induces apoptosis in human hepatocellular carcinoma HepG2 cell via DNA damage, p53 expression, and caspase activation
| Autor: | Haoan Zhao, Sinan Chen, Wei Cao, He Qiong, Ni Cheng |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Carcinoma
Hepatocellular 030309 nutrition & dietetics DNA damage Biophysics Apoptosis Mitochondrion 03 medical and health sciences 0404 agricultural biotechnology Phenols Downregulation and upregulation medicine Humans P53 expression Flavonoids Membrane Potential Mitochondrial Pharmacology Membrane potential 0303 health sciences Chemistry Cell Cycle Liver Neoplasms digestive oral and skin physiology food and beverages Ziziphus Hep G2 Cells Honey 04 agricultural and veterinary sciences Cell Biology Cell cycle medicine.disease 040401 food science Mitochondria Up-Regulation Cell biology Hepatocellular carcinoma DNA Damage Food Science |
| Zdroj: | Journal of Food Biochemistry. 43 |
| ISSN: | 1745-4514 0145-8884 |
| Popis: | Jujube honey, a very popular honey in China, has been shown to own numerous biological properties. However, the anticancer effect and the underlying mechanisms of jujube honey in HepG2 cells have not been investigated to date. This study was designed to evaluate jujube honey-induced apoptosis and its molecular mechanism in HepG2 and the following results were obtained. Firstly, treatment with jujube honey blocked cell cycle progression at the G0/G1 phase, reduced the mitochondrial membrane potential (Δψm), induced DNA damage, and upregulates p53 expression. Secondly, pro-apoptotic proteins Bax and Bad were upregulated and antiapoptotic proteins Bcl-2 and Bcl-xL were downregulated. Lastly, caspase activation and apoptotic death occurred in HepG2 cells. In conclusion, this study showed that jujube honey induces HepG2 cell apoptosis via DNA damage, p53 expression, and caspase activation. PRACTICAL APPLICATIONS: We demonstrate the molecular mechanism by which jujube honey triggers apoptosis in HepG2 cells. This new insight provided useful information on the use of jujube honey as a potential therapeutic and preventive agent. |
| Databáze: | OpenAIRE |
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