Effects of multi-environmental factors on physiological and biochemical responses of large yellow croaker, Larimichthys crocea
| Autor: | Xiongfei Wu, Dan-Li Mu, Cheng Liu, Weiliang Shen, Nian-Gang Guo, Jun-Quan Zhu, Qian-Feng Wang |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Salinity Environmental Engineering Acclimatization Health Toxicology and Mutagenesis ATPase Gene Expression 03 medical and health sciences chemistry.chemical_compound Stress Physiological Lactate dehydrogenase Respiration Animals Environmental Chemistry Larimichthys crocea RNA Messenger biology Succinate dehydrogenase Public Health Environmental and Occupational Health General Medicine General Chemistry Metabolism Hypoxia-Inducible Factor 1 alpha Subunit biology.organism_classification Adaptation Physiological Pollution Perciformes 030104 developmental biology Biochemistry chemistry biology.protein Energy Metabolism Anaerobic exercise |
| Zdroj: | Chemosphere. 184:907-915 |
| ISSN: | 0045-6535 |
| Popis: | Land-based recirculating aquaculture systems (RAS) and cage culture are important methods of Larimichthys crocea production. The effects of environmental factors on physiological and biochemical aspects of L. crocea require clarification. Temperature and salinity are controlled in RAS and directly affect L. crocea growth and survival. To explore optimal parameters, the oxygen consumption rate (R O ), ammonium excretion rate (R N ), and O/N ratio at different temperatures (8, 14, 20, 26, and 32 °C) and salinities (5, 15, 25, and 35‰) were determined. R O , R N , and O/N first increased and then decreased with elevated temperature and salinity, peaking at 26 °C and 25‰, respectively. This suggests that the metabolism of L. crocea was maximal at 26 °C and 25‰ salinity, which promote its growth and survival. Additionally, hypoxia affects cage culture, and has significant effects on enzymatic activities and stress-inducible gene expression. To accelerate the selective breeding of hypoxia-tolerant L. crocea in cage culture, we measured adenosine triphosphatase (ATPase), lactate dehydrogenase (LDH), and succinate dehydrogenase (SDH) activities, and hypoxia-inducing factor 1 (HIF-1) mRNA expression in the myocardium under hypoxia (2.5, 3.5, and 4.5 mg L −1 ). ATPase and SDH activities first decreased and then increased under hypoxia, whereas LDH activity and HIF-1α expression first increased and then decreased. Thus, under hypoxia, the myocardial mitochondria switched from being susceptible to being resistant to injury induced by energy metabolism, and respiration in L. crocea likely converted from aerobic to anaerobic during adaptation. Furthermore, the upregulation of HIF-1α mRNA suggests it has an active role in protection against anoxic damage. |
| Databáze: | OpenAIRE |
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