A new class of cubic SPIONs as a dual-mode T1 and T2 contrast agent for MRI
| Autor: | Emine Ulku Saritas, Zeliha Soran-Erdem, Mustafa Utkur, Oktay Algin, Vivek Sharma, Akbar Alipour, Hilmi Volkan Demir |
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| Přispěvatelé: | Alipour, Akbar, Soran-Erdem, Zeliha, Utkur, Mustafa, Sharma, Vijay Kumar, Algın, Oktay, Sarıtaş, Emine Ülkü, Demir, Hilmi Volkan |
| Rok vydání: | 2017 |
| Předmět: |
Contrast enhancement
Materials science Superparamagnetic iron oxide nanoparticles MRI relaxivity MRI contrast agent media_common.quotation_subject Biomedical Engineering Biophysics Nanoparticle Contrast Media 02 engineering and technology Kidney 030218 nuclear medicine & medical imaging Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Nuclear magnetic resonance Negative Contrast Agent T2 contrast Contrast (vision) Animals Radiology Nuclear Medicine and imaging Magnetite Nanoparticles media_common 021001 nanoscience & nanotechnology Image Enhancement Magnetic Resonance Imaging Dual-mode MRI contrast agents Rats Transmission electron microscopy Models Animal 0210 nano-technology |
| Zdroj: | Magnetic Resonance Imaging |
| ISSN: | 1873-5894 |
| Popis: | Superparamagnetic iron oxide nanoparticles (SPIONs) are widely used as a robust negative contrast agent on conventional MRI. In this study, we (a) synthesized a new class of cubic SPIONs as a dual-mode contrast agent in MRI and (b) showed the in-vivo feasibility of these nanaoparticles as a simultaneous positive and negative contrast agent. Relaxation properties and contrast enhancement analysis of the synthesized SPIONs with two different shapes (cubic vs. spherical) and three different sizes 7 nm, 11 nm, and 14 nm were investigated to evaluate contrast enhancement in-vitro. In-vivo MRI experiments were performed on a 3T MR scanner, where a healthy anesthetized rat was imaged before, and from 20 to 80 min after intravenous injection of 1 mg/kg of contrast agent. Representative transmission electron microscopy (TEM) images of the synthesized nanoparticles reveal that the particles are well dispersed in a solvent and do not aggregate. The in-vitro relaxivity and contrast enhancement analysis show that, among all six SPIONs tested, 11-nm cubic SPIONs possess optimal molar relaxivities and contrast enhancement values, which can shorten the spin-lattice and spin-spin relaxation times, simultaneously. No noticeable toxicity is observed during in-vitro cytotoxicity analysis. In-vivo T1-and T2-weighted acquisitions at 60-min post-injection of 11-nm cubic SPIONs result in 64% and 48% contrast enhancement on the T1-and T2-weighted images, respectively. By controlling the shape and size of SPIONs, we have introduced a new class of cubic SPIONs as a synergistic (dual-mode) MRI contrast agent. 11-nm cubic SPIONs with smaller size and high positive and negative contrast enhancements were selected as a promising candidate for dual-mode contrast agent. Our proof-of-concept MRI experiments on rat demonstrate the in-vivo dual-mode contrast enhancement feasibility of these nanoparticles. We gratefully acknowledge Dr. Gamze Aykut, Department of Molecular Biology and Genetics, Bilkent University, Ankara, Taner Demir and Dr. Volkan Acikel, Bilkent University, Ankara, for their assistance in in-vivo experiments, and all UMRAM staff for providing technical support. The authors also gratefully thank scientific discussion of Dr. Tolga Cukur. We would like to acknowledge Biomaten (METU) for the cytotoxicity experiments. |
| Databáze: | OpenAIRE |
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