Molecular insights into the compatible and incompatible interactions between sugar beet and the beet cyst nematode

Autor: Tim De Meyer, Seyed Bagher Mahmoudi, Rahim Mehrabi, Naser Safaie, Razieh Ghaemi, Tina Kyndt, Bruno Verstraeten, Ebrahim Pourjam
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0106 biological sciences
0301 basic medicine
F-BOX PROTEIN
HETERODERA-SCHACHTII
Plant Science
01 natural sciences
Plant Roots
Transcriptome
chemistry.chemical_compound
Jasmonate
PATHWAY MODULATES ATTRACTIVENESS
lcsh:Botany
Plant defense against herbivory
Arabidopsis thaliana
REAL-TIME PCR
CYSTM domain-containing proteins
Disease Resistance
GENE-EXPRESSION
Genetics
Methyl jasmonate
biology
Sugar beet
food and beverages
ETHYLENE
lcsh:QK1-989
Beta vulgaris
Heterodera schachtii
Research Article
ROOT-KNOT NEMATODES
Genes
Plant
Host-Parasite Interactions
03 medical and health sciences
Ethylene
Animals
Tylenchoidea
TOMATO PLANTS
Plant Diseases
PRODUCTION
fungi
Biology and Life Sciences
Sequence Analysis
DNA
biology.organism_classification
Ascorbic acid
030104 developmental biology
chemistry
ASCORBIC-ACID
ARABIDOPSIS-THALIANA
010606 plant biology & botany
Zdroj: BMC PLANT BIOLOGY
BMC Plant Biology
BMC Plant Biology, Vol 20, Iss 1, Pp 1-16 (2020)
ISSN: 1471-2229
Popis: Background Sugar beet (Beta vulgaris subsp. vulgaris) is an economically important crop that provides nearly one third of the global sugar production. The beet cyst nematode (BCN), Heterodera schachtii, causes major yield losses in sugar beet and other crops worldwide. The most effective and economic approach to control this nematode is growing tolerant or resistant cultivars. To identify candidate genes involved in susceptibility and resistance, the transcriptome of sugar beet and BCN in compatible and incompatible interactions at two time points was studied using mRNA-seq. Results In the susceptible cultivar, most defense-related genes were induced at 4 dai while suppressed at 10 dai but in the resistant cultivar Nemakill, induction of genes involved in the plant defense response was observed at both time points. In the compatible interaction, alterations in phytohormone-related genes were detected. The effect of exogenous application of Methyl Jasmonate and ET-generator ethephon on susceptible plants was therefore investigated and the results revealed significant reduction in plant susceptibility. Genes putatively involved in the resistance of Nemakill were identified, such as genes involved in phenylpropanoid pathway and genes encoding CYSTM domain-containing proteins, F-box proteins, chitinase, galactono-1,4-lactone dehydrogenase and CASP-like protein. Also, the transcriptome of the BCN was analyzed in infected root samples and several novel potential nematode effector genes were found. Conclusions Our data provides detailed insights into the plant and nematode transcriptional changes occurring during compatible and incompatible interactions between sugar beet and BCN. Many important genes playing potential roles in susceptibility or resistance of sugar beet against BCN, as well as some BCN effectors with a potential role as avr proteins were identified. In addition, our findings indicate the effective role of jasmonate and ethylene in enhancing sugar beet defense response against BCN. This research provides new molecular insights into the plant-nematode interactions that can be used to design novel management strategies against BCN.
Databáze: OpenAIRE
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