The salivary transcriptome of Anopheles gambiae (Diptera: Culicidae) larvae: A microarray-based analysis
| Autor: | T. Moroz, A. Heyland, Paul J. Linser, M. Neira Oviedo, L. VanEkeris, José M. C. Ribeiro |
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| Rok vydání: | 2008 |
| Předmět: |
Saliva
Anopheles gambiae Molecular Sequence Data Biochemistry Salivary Glands Article Transcriptome Anopheles medicine Animals Amino Acid Sequence Molecular Biology Defensin Phylogeny Oligonucleotide Array Sequence Analysis biology Salivary gland Base Sequence Microarray analysis techniques Gene Expression Profiling fungi biology.organism_classification Molecular biology Gene expression profiling medicine.anatomical_structure Insect Science Larva Insect Proteins DNA microarray Sequence Alignment |
| Zdroj: | Insect biochemistry and molecular biology. 39(5-6) |
| ISSN: | 1879-0240 |
| Popis: | In spite of the many recent developments in the field of vector sialomics, the salivary glands of larval mosquitoes have been largely unexplored. We used whole-transcriptome microarray analysis to create a gene-expression profile of the salivary gland tissue of fourth-instar Anopheles gambiae larvae, and compare it to the gene-expression profile of a matching group of whole larvae. We identified a total of 221 probes with expression values that were (a) significantly enriched in the salivary glands, and (b) sufficiently annotated as to allow the prediction of the presence/absence of signal peptides in their corresponding gene products. Based on available annotation of the protein sequences associated with these probes, we propose that the main roles of larval salivary secretions include: (a) immune response, (b) mouthpart lubrication, (c) nutrient metabolism, and (d) xenobiotic detoxification. Other highlights of the study include the cloning of a transcript encoding a previously unknown salivary defensin (AgDef5), the confirmation of mucus secretion by the larval salivary glands, and the first report of salivary lipocalins in the Culicidae. |
| Databáze: | OpenAIRE |
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