Characterization of H4: a mouse T lymphocyte activation molecule functionally associated with the CD3/T cell receptor
| Autor: | Sara Bonissoni, José M. Rojo, Henrik Wolff, Manuela Bragardo, Umberto Dianzani, Charles A. Janeway, Donatella Buonfiglio, Pilar Portolés, Valter Redoglia |
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| Rok vydání: | 1996 |
| Předmět: |
Antigens
Differentiation T-Lymphocyte CD3 T cell T-Lymphocytes Immunology Biology Lymphocyte Activation Immunophenotyping 03 medical and health sciences Mice 0302 clinical medicine Cricetulus Antigen Cricetinae medicine Immunology and Allergy Cytotoxic T cell Animals IL-2 receptor 030304 developmental biology 0303 health sciences ZAP70 T-cell receptor Antibodies Monoclonal Protein-Tyrosine Kinases Molecular biology Precipitin Tests medicine.anatomical_structure Receptor-CD3 Complex Antigen T-Cell biology.protein CD8 030215 immunology Signal Transduction |
| Zdroj: | European journal of immunology. 26(11) |
| ISSN: | 0014-2980 |
| Popis: | The monoclonal antibody C398.4A was produced by immunizing Armenian hamsters with the mouse T cell clone D10.G4.1. It recognizes a molecule selectively expressed by activated mouse T cells and was named H4. H4 is expressed on the T cell surface about 24 h after activation and peaks at day 7. By contrast, it is not expressed by resting or activated B cells, macrophages, or fibroblasts. It is also expressed by CD4 or CD8 single-positive mature thymocytes. Immunoprecipitation showed that H4 is a disulfide-linked dimer, precipitating as a broad band at about 50-65 kDa under nonreducing conditions and at 25 and 29 kDa under reducing conditions. Deglycosylation of the reduced H4 by N-glycanase gave rise to a single band of about 21 kDa, suggesting that the two chains may be differentially glycosylated forms of the same protein. The H4 expression pattern and biochemical features, together with cross-blocking, co-capping, co-modulation, and immunoprecipitation preclearing experiments showed that H4 is different from other known co-stimulatory molecules such as CD69, CD2, Ly-6, CD25, OX-40, Mac-1 and LFA-1. By in vitro kinase assay, H4 was found to co-precipitate a tyrosine kinase activity that phosphorylated substrates of about 29 and 25 kDa. Co-modulation and co-capping experiments showed that H4 is physically associated with the CD3/T cell receptor. These data suggest that H4 may function as a T cell-specific co-stimulatory molecule and play a role in the T cell response when the activation stimulus is limited either because the antigen is only available in low concentration or has a low agonistic activity. |
| Databáze: | OpenAIRE |
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