Immobilization of papain enzyme on a hybrid support containing zinc oxide nanoparticles and chitosan for clinical applications

Autor: Raúl Fangueiro, Lizia M. O. Gonçalves, Michel Muálem de Moraes Alves, Ruanna D. S. Ferreira, Welter Cantanhêde, Aurileide M. B. F. Soares, Jefferson Mendes de Souza, Fernando Aécio A. Carvalho, Anderson Nogueira Mendes
Přispěvatelé: Universidade do Minho
Rok vydání: 2020
Předmět:
Male
Ciências Agrárias::Biotecnologia Agrária e Alimentar
Biotecnologia Agrária e Alimentar [Ciências Agrárias]
Polymers and Plastics
Immobilized enzyme
Nanoparticle
chemistry.chemical_element
Biocompatible Materials
02 engineering and technology
Zinc
010402 general chemistry
01 natural sciences
Chitosan
Mice
chemistry.chemical_compound
Biotecnologia Médica [Ciências Médicas]
Papain
Zinc oxide
Materials Chemistry
Animals
Enzyme immobilization
Cells
Cultured
chemistry.chemical_classification
Mice
Inbred BALB C
Science & Technology
Organic Chemistry
technology
industry
and agriculture
Enzymes
Immobilized
021001 nanoscience & nanotechnology
Clinical application
0104 chemical sciences
Enzyme
chemistry
Nanoparticles
Ciências Médicas::Biotecnologia Médica
Female
Hybrid materials
0210 nano-technology
Hybrid material
Nuclear chemistry
Zdroj: Repositório Científico de Acesso Aberto de Portugal
Repositório Científico de Acesso Aberto de Portugal (RCAAP)
instacron:RCAAP
ISSN: 0144-8617
DOI: 10.1016/j.carbpol.2020.116498
Popis: A new hybrid bionanomaterial composed of zinc oxide nanoparticles (ZnO NPs) and chitosan was constructed after enzymatic immobilization of papain for biomedical applications. In this work, we report the preparation and characterization steps of this bionanomaterial and its biocompatibility in vitro. The properties of the immobilized papain system were investigated by transmission electron microscopy, zeta potential, DLS, UV-vis absorption spectroscopy, FTIR spectroscopy, and X-ray diffraction. The prepared bionanomaterial exhibited a nanotriangular structure with a size of 150 nm and maintained the proteolytic activity of papain. In vitro analyses demonstrated that the immobilized papain system decreased the activation of phagocytic cells but did not induce toxicity. Based on the results obtained, we suggest that the novel bionanomaterial has great potential in biomedical applications in diseases such as psoriasis and wounds.
Financial support from CNPq (310678/2014-5), FAPEPI and CAPES (Rede nBioNet) is gratefully acknowledged
Databáze: OpenAIRE
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