Evaluation of porcine valves prepared by dye-mediated photooxidation
| Autor: | Mark Moore, Richard E. Phillips, Virginia M. Walley, Paul J. Hendry, Brian K. McIlroy |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Pulmonary and Respiratory Medicine
Radiation-Sensitizing Agents Pathology medicine.medical_specialty Tissue Fixation Light Swine Prosthesis Design Rats Sprague-Dawley Fixatives Fresh Tissue In vivo medicine Animals Pericardium Heart valve Coloring Agents Bioprosthesis Heart Valve Prosthesis Implantation Minerals Sheep business.industry Calcinosis Proteins Phosphorus medicine.disease In vitro Rats Resorption medicine.anatomical_structure Solubility Evaluation Studies as Topic Glutaral Heart Valve Prosthesis Calcium Cattle Surgery Cardiology and Cardiovascular Medicine business Oxidation-Reduction Follow-Up Studies Calcification Explant culture |
| Zdroj: | The Annals of Thoracic Surgery. 66:S245-S248 |
| ISSN: | 0003-4975 |
| DOI: | 10.1016/s0003-4975(98)01118-7 |
| Popis: | Background . Previous studies demonstrated that dye-mediated photooxidation can stabilize bovine pericardium. Here, photooxidized porcine valve cusp and root tissue were assessed in comparison to fresh and glutaraldehyde-treated samples. Methods and Results . In an in vitro tissue solubility test, both photooxidized and glutaraldehyde-treated tissues were resistant to protein extraction compared to fresh tissue. A rat subcutaneous model was used to test in vivo stability and calcification potential. In this study, four of the six fresh leaflets were not visible because of resorption while both photooxidized and glutaraldehyde-treated tissues were biostable. Mineral contents of the rat explants were much lower for both fresh and photooxidized leaflets when compared with glutaraldehyde-treated leaflets. Also, the aortic root calcified whether treated or not with the most mineral being associated with glutaraldehyde-treated root. Analysis of photooxidized porcine valves explanted from the mitral position in sheep indicated a material that was biostable and contained only minor calcification, perhaps due to deformed stents. Conclusions . Porcine valve tissue treated by dye-mediated photooxidation is biostable and resistant to calcification, and has potential for use in heart valve bioprostheses. |
| Databáze: | OpenAIRE |
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