Binase treatment increases interferon sensitivity and apoptosis in SiHa cervical carcinoma cells by downregulating E6 and E7 human papilloma virus oncoproteins
| Autor: | Irina Yu. Petrushanko, Vladimir A. Mitkevich, K. M. Burnysheva, Alexey A. Schulga, Alexei A. Adzhubei, Peter M. Chumakov, Alexander A. Makarov |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
HPV Viral protein Biology medicine.disease_cause Flow cytometry 03 medical and health sciences Interferon Cervical carcinoma medicine Ribonuclease medicine.diagnostic_test apoptosis viral oncoproteins Virology cytotoxic RNase female genital diseases and pregnancy complications 030104 developmental biology Oncology Apoptosis Cancer cell biology.protein Cancer research cancer cells Intracellular medicine.drug Research Paper |
| Zdroj: | Oncotarget |
| ISSN: | 1949-2553 |
| Popis: | In this study, we determined whether binase, a ribonuclease from Bacillus pumilus, increases interferon sensitivity and apoptosis in SiHa cervical cancer cells infected with high-risk human papilloma virus (HPV) strain 16. Binase treatment increased SiHa cell apoptosis in a time- and concentration-dependent manner, as determined by flow cytometry, WST tests and real time xCelligence cell index analysis. Binase-treated SiHa cells showed reduced expression of E6 and E7 viral oncoproteins and increased expression of their intracellular targets, p53 and pRb. Combined treatment with binase and IFNα2b enhanced the interferon sensitivity of HPV-positive SiHa cells. By contrast, combined treatment with binase and IFNα2b in HPV-negative C33A cervical cancer cells, which do no expess E6 and E7, elicited no changes in interferon sensitivity or p53 and pRb expression. These findings suggest binase enhances interferon sensitivity and apoptosis in HPV-positive SiHa cervical cancer cells by suppressing E6 and E7 viral protein expression. |
| Databáze: | OpenAIRE |
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