Characterization, Occurrence, and Molecular Cloning of a Lectin fromGrifola frondosa: Jacalin-Related Lectin of Fungal Origin
| Autor: | Yoshiho Nagata, Masako Yamashita, Junko Akabane, Kozo Nishibori, Futoshi Sumisa, Koichi Uehara, Hiroaki Honda, Akihiro Saito, Yasuo Oodaira |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
DNA
Complementary Molecular Sequence Data Sequence Homology Biology Molecular cloning Applied Microbiology and Biotechnology Biochemistry Analytical Chemistry Lectins Databases Genetic Amino Acid Sequence Cloning Molecular DNA Fungal Molecular Biology Peptide sequence Grifola frondosa chemistry.chemical_classification Molecular mass Basidiomycota Organic Chemistry Protein primary structure Lectin Hemagglutination Tests General Medicine Chromatography Ion Exchange Amino acid Molecular Weight chemistry Jacalin biology.protein Carbohydrate Metabolism Electrophoresis Polyacrylamide Gel Plant Lectins Grifola Biotechnology |
| Zdroj: | Bioscience, Biotechnology, and Biochemistry. 69:2374-2380 |
| ISSN: | 1347-6947 0916-8451 |
| DOI: | 10.1271/bbb.69.2374 |
| Popis: | A lectin named GFL was isolated from the fruiting body of the basidiomycete mushroom Grifola frondosa, which belongs to Aphyllophorales. The lectin had a molecular mass of 24 kDa on SDS-PAGE. The hemagglutinating activity of GFL was not inhibited by any monosaccharide, and inhibited only by porcine stomach mucin so far as tested. The occurrence of GFL was studied at three stages during fruiting body formation. The largest quantity of hemagglutinating activity was found in the fruiting body, and lesser amounts in the mycelial mat and the primordium. The 24-kDa band of GFL was found at all three stages, and the band-intensity corresponded to the level of activity in each sample. By cloning and sequencing the GFL-cDNA, the primary structure of this lectin was determined. GFL is composed of 181 amino acids, having no signal peptide. The amino acid sequence was found to be homologous to those of so-called jacalin-related plant lectins, suggesting that GFL is the first example of a jacalin-related lectin of fungal origin. |
| Databáze: | OpenAIRE |
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