Growth, ethanol production, and inulinase activity on various inulin substrates by mutant Kluyveromyces marxianus strains NRRL Y-50798 and NRRL Y-50799
| Autor: | Marjorie A. Jones, Kenneth M. Bischoff, Mitchell R. Lindquist, Stephen R. Hughes, Elby J. Cox, Nasib Qureshi, Joshua M. Jarodsky, Juan Carlos López-Núñez, Adam Erickson, Siqing Liu, Luz Ángela Galindo-Leva, Eric C. Hoecker |
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| Jazyk: | angličtina |
| Předmět: |
0106 biological sciences
0301 basic medicine Inulinase activity Glycoside Hydrolases Inulin Bioengineering Coffee waste fermentation Biorefinery platform 01 natural sciences Coffee Applied Microbiology and Biotechnology 03 medical and health sciences chemistry.chemical_compound Kluyveromyces Kluyveromyces marxianus 010608 biotechnology Ethanol fuel Food science Inulinase Ethanol biology biology.organism_classification Bioenergy/Biofuels/Biochemicals Culture Media 030104 developmental biology Glucose chemistry Biochemistry Biofuel Biofuels Mutation Biotechnology |
| Zdroj: | Journal of Industrial Microbiology & Biotechnology |
| ISSN: | 1367-5435 |
| DOI: | 10.1007/s10295-016-1771-5 |
| Popis: | Economically important plants contain large amounts of inulin. Disposal of waste resulting from their processing presents environmental issues. Finding microorganisms capable of converting inulin waste to biofuel and valuable co-products at the processing site would have significant economic and environmental impact. We evaluated the ability of two mutant strains of Kluyveromyces marxianus (Km7 and Km8) to utilize inulin for ethanol production. In glucose medium, both strains consumed all glucose and produced 0.40 g ethanol/g glucose at 24 h. In inulin medium, Km7 exhibited maximum colony forming units (CFU)/mL and produced 0.35 g ethanol/g inulin at 24 h, while Km8 showed maximum CFU/mL and produced 0.02 g ethanol/g inulin at 96 h. At 24 h in inulin + glucose medium, Km7 produced 0.40 g ethanol/g (inulin + glucose) and Km8 produced 0.20 g ethanol/g (inulin + glucose) with maximum CFU/mL for Km8 at 72 h, 40 % of that for Km7 at 36 h. Extracellular inulinase activity at 6 h for both Km7 and Km8 was 3.7 International Units (IU)/mL. Electronic supplementary material The online version of this article (doi:10.1007/s10295-016-1771-5) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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