A three-dimensional hydroxyapatite/polyacrylonitrile composite scaffold designed for bone tissue engineering
| Autor: | Lin Jin, Shuyi Wu, Zhenling Wang, Jieda Wang, Yan Li, Leiyan Zou |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Scaffold
Materials science General Chemical Engineering Regeneration (biology) Composite number Polyacrylonitrile 02 engineering and technology General Chemistry 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Bone tissue engineering Electrospinning 0104 chemical sciences chemistry.chemical_compound chemistry Cell culture Composite scaffold 0210 nano-technology Biomedical engineering |
| Zdroj: | RSC Advances. 8:1730-1736 |
| ISSN: | 2046-2069 |
| DOI: | 10.1039/c7ra12449j |
| Popis: | In recent years, various composite scaffolds based on hydroxyapatite have been developed for bone tissue engineering. However, the poor cell survival micro-environment is still the major problem limiting their practical applications in bone repairing and regeneration. In this study, we fabricated a class of fluffy and porous three-dimensional composite fibrous scaffolds consisting of hydroxyapatite and polyacrylonitrile by employing an improved electrospinning technique combined with a bio-mineralization process. The fluffy structure of the hydroxyapatite/polyacrylonitrile composite scaffold ensured the cells would enter the interior of the scaffold and achieve a three-dimensional cell culture. Bone marrow mesenchymal stem cells were seeded into the scaffolds and cultured for 21 days in vitro to evaluate the response of cellular morphology and biochemical activities. The results indicated that the bone marrow mesenchymal stem cells showed higher degrees of growth, osteogenic differentiation and mineralization than those cultured on the two-dimensional hydroxyapatite/polyacrylonitrile composite membranes. The obtained results strongly supported the fact that the novel three-dimensional fluffy hydroxyapatite/polyacrylonitrile composite scaffold had potential application in the field of bone tissue engineering. |
| Databáze: | OpenAIRE |
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