Self-Collected Oral Fluid Saliva Is Insensitive Compared With Nasal-Oropharyngeal Swabs in the Detection of Severe Acute Respiratory Syndrome Coronavirus 2 in Outpatients
| Autor: | Manabe, Yukari C, Reuland, Carolyn, Yu, Tong, Azamfirei, Razvan, Hardick, Justin P, Church, Taylor, Brown, Diane M, Sewell, Thelio T, Antar, Annuka, Blair, Paul W, Heaney, Chris D, Pekosz, Andrew, Thomas, David L, Cox, Andrea, Keller, Sara, Keruly, Jeanne, Klein, Sabra, Mehta, Shruti, Mostafa, Heba, Pisanic, Nora, Sauer, Lauren, Tornheim, Jeffrey, Townsend, Jennifer, Armstrong, Derek, Bachu, Vismaya, Barnaba, Brittany, Charles, Curtisha, Dai, Weiwei, Ganesan, Abhinaya, Holden, Jeffrey, Jang, Minyoung, Johnstone, J R, Kruczynski, Kate, Kusemiju, Oyinkansola, Lambrou, Anastasia, Li, Lucy, Littlefield, Kirsten, Park, Han-Sol, Tuchler, Amanda, Montana, Manuela Plazas, Prizzi, Michelle, Ursin, Rebecca |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Saliva Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) 030106 microbiology medicine.disease_cause 03 medical and health sciences 0302 clinical medicine Internal medicine medicine Major Article Sampling (medicine) 030212 general & internal medicine Nose Coronavirus saliva business.industry SARS-CoV-2 COVID-19 medicine.anatomical_structure AcademicSubjects/MED00290 Infectious Diseases Specimen collection Oncology Ambulatory Cohort outpatient business |
| Zdroj: | Open Forum Infectious Diseases |
| ISSN: | 2328-8957 |
| Popis: | Background Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic control will require widespread access to accurate diagnostics. Salivary sampling circumvents swab supply chain bottlenecks, is amenable to self-collection, and is less likely to create an aerosol during collection compared with the nasopharyngeal swab. Methods We compared real-time reverse-transcription polymerase chain reaction Abbott m2000 results from matched salivary oral fluid (gingival crevicular fluid collected in an Oracol device) and nasal-oropharyngeal (OP) self-collected specimens in viral transport media from a nonhospitalized, ambulatory cohort of coronavirus disease 2019 (COVID-19) patients at multiple time points. These 2 sentences should be at the beginning of the results. Results There were 171 matched specimen pairs. Compared with nasal-OP swabs, 41.6% of the oral fluid samples were positive. Adding spit to the oral fluid percent collection device increased the percent positive agreement from 37.2% (16 of 43) to 44.6% (29 of 65). The positive percent agreement was highest in the first 5 days after symptoms and decreased thereafter. All of the infectious nasal-OP samples (culture positive on VeroE6 TMPRSS2 cells) had a matched SARS-CoV-2 positive oral fluid sample. Conclusions In this study of nonhospitalized SARS-CoV-2-infected persons, we demonstrate lower diagnostic sensitivity of self-collected oral fluid compared with nasal-OP specimens, a difference that was especially prominent more than 5 days from symptom onset. These data do not justify the routine use of oral fluid collection for diagnosis of SARS-CoV-2 despite the greater ease of collection. It also underscores the importance of considering the method of saliva specimen collection and the time from symptom onset especially in outpatient populations. |
| Databáze: | OpenAIRE |
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