To knockout in 129 or in C57BL/6: that is the question

Autor:	Eunju Seong, Colin L Stewart, Margit Burmeister, Thomas L. Saunders
Rok vydání:	2004
Předmět:	Mice Knockout Genetics C57BL/6 Microinjections Mouse strain Stem Cells Strain (biology) Gene Transfer Techniques Cell Differentiation Biology biology.organism_classification Null allele Embryonic stem cell Cell biology Mice Inbred C57BL Mice Blastocyst Germ Cells Backcrossing Animals Cells Cultured
Zdroj:	Trends in Genetics. 20:59-62
ISSN:	0168-9525
DOI:	10.1016/j.tig.2003.12.006
Popis:	Traditionally, knockout experiments are performed in ES cells derived from the 129 mouse strain, followed by backcrossing with the more robust C57BL/6 strain. C57BL/6-derived ES cells have only occasionally been used in this process. We compared C57BL/6- with 129-derived ES cells directly and reviewed the literature. We found that, although some steps are less efficient, the advantages of C57BL/6 mice more than compensate for these drawbacks.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::edaa22207855c9507b0b7a1978814921 https://doi.org/10.1016/j.tig.2003.12.006 Zobrazit plný text záznamu Full Text from ScienceDirect