The application of enzyme-linked immunosorbent assays (ELISA) to neuropeptides
| Autor: | Betty Wolfson, Leonard G. Davis, Stephen T. Toy, Alice M. Callahan, Frank Baldino, Robert W. Manning |
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| Rok vydání: | 1985 |
| Předmět: |
Time Factors
Immunocytochemistry Neuropeptide Enzyme-Linked Immunosorbent Assay Immunoenzyme Techniques Nitrophenols chemistry.chemical_compound Organophosphorus Compounds medicine Animals Chromatography High Pressure Liquid Neurotensin Brain Chemistry chemistry.chemical_classification medicine.diagnostic_test biology Chemistry Chromogenic Immune Sera General Neuroscience Alkaline Phosphatase Molecular biology Rats Titer Enzyme Immunoassay biology.protein Rabbits Antibody |
| Zdroj: | Journal of Neuroscience Methods. 14:15-23 |
| ISSN: | 0165-0270 |
| DOI: | 10.1016/0165-0270(85)90111-6 |
| Popis: | Procedures are presented for routine evaluation of antibody specificity, titre, and quantitation of antigen levels in tissue extracts without the use of radiolabeled probes. A colorimetric, enzyme-linked immunosorbent assay (ELISA) is described for general use with neuropeptides, using neurotensin as a primary example. These assays use rabbit anti-neurotensin immune serum which is colorimetrically identified after combination with an alkaline phosphatase-conjugated, affinity purified, goat anti-rabbit IgG and reaction with the chromogenic substrate, p-nitrophenyl phosphate. Because the principle of these methods can be adapted for use with various proteins and neuropeptides, they should find widespread applicability in neurobiology. |
| Databáze: | OpenAIRE |
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