Denuded human amniotic membrane seeding bone marrow stromal cells as an effective composite matrix stimulates axonal outgrowth of rat neural cortical cells in vitro
| Autor: | Xiao ping Xu, Hong sheng Liang, Tao Liang, Peng Liang, En zhong Liu, Ye Xu, Jia ning Wu |
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| Rok vydání: | 2008 |
| Předmět: |
Pathology
medicine.medical_specialty Stromal cell Growth Cones Matrix (biology) Transplantation Autologous Basement Membrane law.invention Confocal microscopy law Medicine Animals Humans Amnion Cells Cultured Bone Marrow Transplantation Basement membrane Neuronal Plasticity Tissue Engineering business.industry Cell Differentiation Recovery of Function In vitro Coculture Techniques Cell biology Extracellular Matrix Nerve Regeneration Rats Transplantation medicine.anatomical_structure Surgery Brain Damage Chronic Female Neurology (clinical) Bone marrow Stem cell Stromal Cells business |
| Zdroj: | Acta neurochirurgica. 151(9) |
| ISSN: | 0942-0940 |
| Popis: | Previous studies have shown that axonal outgrowth in the damaged central nervous system is closely related to the local microenvironment. Transplantation of bone marrow stromal cells (BMSC) or BMSC with some biomaterials has been used to treat various central nervous system diseases with some success. In the current study, we investigated if BMSC on denuded human amniotic membrane (DhAM) as a composite matrix could stimulate axonal outgrowth or not. After completely removing the cells on the amniotic membrane with a tryptic and mechanical approach, we seeded BMSC on it. The MTS was applied to test the cytotoxicity of DhAM compared with PLGA and PLL. The morphology of the BMSC was observed by light, electronic and laser confocal microscopy. We also used four kinds of substance (PLL, DhAM, BMSC + PLL, BMSC + DhAM) to coculturing with the cortical neurons. Finally, the lengths of axons in each group were studied using the positive axon-specific marker NF–H. The DhAM was devoid of cellular components and only its intact basement membrane was left. BMSC grew on the substrate and proliferated with a flat to fusiform morphology. In the MTS test, the results indicated that BMSC cultured in DhAM extract had a high survival rate (> 80%). Moreover, the cortical neural axons in the experimental group (BMSC + DhAM) were longer (287.37 ± 12.72 μm) than in the other groups (P |
| Databáze: | OpenAIRE |
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