Evaluation of serological diagnostic tests of human brucellosis for prevention and control in Mexico
| Autor: | Sergio Pastén-Sánchez, Jesús Felipe González-Roldán, Irma López-Martínez, Fernando Vargas-Pino, Berenice Salgado-Jiménez, Jorge Membrillo-Hernández, Carmen Guzmán-Bracho, Irma Hernández-Monroy, José Alberto Díaz-Quiñonez, Liz G Beltrán-Parra, Demetrio Rodríguez |
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| Rok vydání: | 2020 |
| Předmět: |
Adult
Male 0301 basic medicine Microbiology (medical) medicine.medical_specialty 030106 microbiology Sensitivity and Specificity Brucellosis Serology Lateral flow test Young Adult 03 medical and health sciences 0302 clinical medicine Cohen's kappa Risk Factors Internal medicine Humans Medicine Public Health Surveillance Serologic Tests 030212 general & internal medicine Mexico Human brucellosis Serodiagnoses business.industry Zoonosis Disease Management Reproducibility of Results Diagnostic test General Medicine Middle Aged medicine.disease Brucella Infectious Diseases Female business Algorithms Follow-Up Studies |
| Zdroj: | European Journal of Clinical Microbiology & Infectious Diseases. 39:575-581 |
| ISSN: | 1435-4373 0934-9723 |
| DOI: | 10.1007/s10096-019-03760-3 |
| Popis: | Brucellosis is a zoonosis mainly present in developing countries. The WHO reports 500,000 new cases every year. From 2012 to 2016, 13,677 cases were reported in Mexico, with 2.00 to 2.64 rate per 100,000 inhabitants. To analyze the diagnostic algorithm of brucellosis in Mexico, we compared the commercial laboratory tests ELISA, Brucellacapt®, and lateral flow test (LFT) in a study of 473 individuals from two endemic Mexican populations. All patients were treated in first-level medical units for presenting brucellosis compatible symptoms and without a history of the disease. Clinical-epidemiological information was gathered and initial serum samples were obtained to react with anti-Brucella antibodies; subsequent samples were collected at follow-up treatment visits. Using the Rose Bengal screening, we found 165 negative samples and 308 positive reactive samples, of which 222 cases were confirmed and 234 were positive on at least one marker (IgG or IgM) or LFT. When Brucellacapt® was used, similar results to those observed with the conventional algorithm were found as judged by the Cohen's kappa coefficient (κ) (0.813, 95% CI 0.7788-0.8472). Similar κ indices between conventional algorithm and ELISA pair were found, 0.7038 (95% CI 0.6555-0.7521), representing high similarity between both groups of diagnosis. We conclude that conventional serodiagnoses, Brucellacapt® and LFT, presented inconclusive results and poor correlation between them. By contrast, ELISA test pair (IgG + IgM) presented high correlation with the conventional algorithm and greater capacity for correct positive and negative classification. |
| Databáze: | OpenAIRE |
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