Dynamic Changes in the Ability to Release Neutrophil ExtraCellular Traps in the Course of Childhood Acute Leukemias

Autor: Urszula Demkow, Malgorzata Wachowska, Eliza Glodkowska-Mrowka, Michal Przemyslaw Pruchniak, Edyta Ulińska, Olga Ciepiela, Piotr Mrowka, Magdalena Ostafin
Rok vydání: 2021
Předmět:
Neutrophils
Extracellular Traps
lcsh:Chemistry
0302 clinical medicine
AML
hemic and lymphatic diseases
Medicine
Child
lcsh:QH301-705.5
Cells
Cultured
Spectroscopy
education.field_of_study
Acute leukemia
Leukemia
Childhood Acute Myeloid Leukemia
neutrophil
General Medicine
Computer Science Applications
N-Formylmethionine Leucyl-Phenylalanine
Calcium Ionophores
Child
Preschool
030220 oncology & carcinogenesis
Acute Disease
Absolute neutrophil count
Tetradecanoylphorbol Acetate
Adolescent
Population
neutrophil extracellular traps
childhood acute myeloid leukemia
Neutropenia
Article
Catalysis
Inorganic Chemistry
03 medical and health sciences
Humans
neutropenia
Physical and Theoretical Chemistry
education
Molecular Biology
Childhood Acute Lymphoblastic Leukemia
business.industry
Organic Chemistry
Infant
NETs
Neutrophil extracellular traps
medicine.disease
Immunity
Innate
lcsh:Biology (General)
lcsh:QD1-999
childhood acute lymphoblastic leukemia
Immunology
ALL
business
030215 immunology
Zdroj: International Journal of Molecular Sciences
Volume 22
Issue 2
International Journal of Molecular Sciences, Vol 22, Iss 821, p 821 (2021)
ISSN: 1422-0067
DOI: 10.3390/ijms22020821
Popis: Acute leukemias, the most common cancers in children, are characterized by excessive proliferation of malignant progenitor cells. As a consequence of impaired blood cell production, leukemia patients are susceptible to infectious complications&mdash
a major cause of non-relapse mortality. Neutrophil extracellular traps (NETs) are involved in various pathologies, from autoimmunity to cancer. Although aberrant NETs formation may be partially responsible for immune defects observed in acute leukemia, still little is known on the NET release in the course of leukemia. Here, we present the first comprehensive evaluation of NETs formation by neutrophils isolated from children with acute leukemia in different stages of the disease and treatment stimulated in vitro with phorbol 12-myristate 13-acetate (PMA), N-formyl-methionyl-leucyl-phenylalanine (fMLP), and calcium ionophore (CI). NETs release was measured using quantitative fluorescent method and visualized microscopically. In this setting, NETs release was significantly impaired in leukemic children both at the diagnosis and during the treatment, and full restoration of neutrophil function was achieved only after successful completion of the leukemia treatment. We suggest that neutrophil function impairment may result from both disease- and treatment-related factors. In this context, deficient innate immune response observed in acute leukemia patients may be present regardless of neutrophil count and contribute to secondary immunodeficiency observed in this population.
Databáze: OpenAIRE
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