Degradation of 2,4,6-trinitrotoluene by P. aeruginosa and characterization of some metabolites
| Autor: | Gulcihan Guzeldag, Melis Sumengen Ozdenefe, Sadik Dinçer, Fatih Matyar, Hatice Aysun Mercimek, Aysenur Ozsavli, Fikret Buyukkaya Kayis, Afet Arkut |
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| Přispěvatelé: | Çukurova Üniversitesi |
| Rok vydání: | 2015 |
| Předmět: |
Time Factors
lcsh:QR1-502 Microbiology High-performance liquid chromatography lcsh:Microbiology Gas Chromatography-Mass Spectrometry Nitroaromatic explosives Pseudomonas sp chemistry.chemical_compound Bioremediation Biotransformation TNT Environmental Microbiology Media Technology nitroaromatic explosives Trinitrotoluene Nitrite Chromatography High Pressure Liquid Aniline Compounds Chromatography biology Chemistry musculoskeletal system biology.organism_classification Thin-layer chromatography Culture Media Dinitrobenzenes Pseudomonas aeruginosa Chromatography Thin Layer biotransformation Gas chromatography–mass spectrometry Metabolic Networks and Pathways Bacteria |
| Zdroj: | Brazilian Journal of Microbiology v.46 n.1 2015 Brazilian Journal of Microbiology Sociedade Brasileira de Microbiologia (SBM) instacron:SBM Brazilian Journal of Microbiology, Vol 46, Iss 1, Pp 103-111 (2015) Brazilian Journal of Microbiology, Volume: 46, Issue: 1, Pages: 103-111, Published: MAR 2015 |
| ISSN: | 1678-4405 |
| DOI: | 10.1590/s1517-838246120140026 |
| Popis: | PubMedID: 26221094 Degradation of 2,4,6-trinitrotoluene (TNT), a nitroaromatic explosive found in the soil and ground water, was investigated using Pseudomonas aeruginosa in in vitro experiments. Biodegradable abilitiy of this bacteria was performed with 50 and 75 mg L-1 TNT concentrations in a defined liquid medium for 96 h time period. Treatment of TNT in supernatant samples taken at 0, 6, 12, 24, 48, 72 and 96 h from agitated vessels was followed by reverse-phase high-performance liquid chromatography (HPLC). In cultures supplemented with 50 and 75 mgL-1 TNT, after 96 h of incubation 46% and 59% reduction were detected respectively. Two metabolites as degradation intermediates with nitrite release into the medium, 2,4-dinitrotoluene (2,4-DNT) and 4-aminodinitrotoluene (4-ADNT), were elucidated by thin layer chromatography (TLC) and gas chromatography-mass spectrometry (GCMS). These findings clearly indicate that Pseudomonas aeruginosa can be used in bioremediation of TNT contaminated sites. © 2015, Sociedade Brasileira de Microbiologia. |
| Databáze: | OpenAIRE |
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