Directed cell growth on protein-functionalized hydrogel surfaces
Autor: | William Shain, Matthew R. Hynd, John P. Frampton, Natalie Dowell-Mesfin, James N. Turner |
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Rok vydání: | 2006 |
Předmět: |
Surface Properties
Cell Culture Techniques Biocompatible Materials Pyridinium Compounds Cell morphology Article Laminin Animals Biotinylation Neurons biology Chemistry Cell growth General Neuroscience Proteins Hydrogels Molecular biology Fibronectins Rats Fibronectin Quaternary Ammonium Compounds Kinetics Microcontact printing Self-healing hydrogels biology.protein Biophysics Surface modification Astroglioma Cell Division |
Zdroj: | Journal of neuroscience methods. 162(1-2) |
ISSN: | 0165-0270 |
Popis: | Biochemical surface modification has been used to direct cell attachment and growth on a biocompatible gel surface. Acrylamide-based hydrogels were photo-polymerized in the presence of an acroyl-streptavidin monomer to create planar, functionalized surfaces capable of binding biotin-labelled proteins. Soft protein lithography (microcontact printing) of proteins was used to transfer the biotinylated extracellular matrix proteins, fibronectin and laminin, and the laminin peptide biotin-IKVAV, onto modified surfaces. As a biological assay, we plated LRM55 astroglioma and primary rat hippocampal neurons on patterned hydrogels. We found both cell types to selectively adhere to areas patterned with biotin-conjugated proteins. Fluorescence and bright-field modes of microscopy were used to assess cell attachment and cell morphology on modified surfaces. LRM55 cells were found to attach to protein-stamped regions of the hydrogel only. Neurons exhibited significant neurite extension after 72 hr in vitro, and remained viable on protein stamped areas for more than 4 weeks. Patterned neurons developed functionally active synapses, as measured by uptake of the dye FM 1-43FX. Results from this study suggest that hydrogel surfaces can be patterned with multiple proteins to direct cell growth and attachment. |
Databáze: | OpenAIRE |
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