ATP Induces IL-1β Secretion in Neisseria gonorrhoeae-Infected Human Macrophages by a Mechanism Not Related to the NLRP3/ASC/Caspase-1 Axis

Autor: Rolando Vernal, Claudio A. Perez, Caroll J. Beltrán, Gisselle Escobar, Alejandro Escobar, Sergio Arancibia, Claudio Acuña-Castillo, Killen Garciá, Pablo A. Mendoza, Paula I. Rodas
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
EXPRESSION
Article Subject
Immunology
Interleukin-1beta
Caspase 1
Organic Anion Transporters
Sodium-Dependent
EXTRACELLULAR ATP
Apoptosis
Real-Time Polymerase Chain Reaction
NLRP3 INFLAMMASOME
KAPPA-B ACTIVATION
03 medical and health sciences
chemistry.chemical_compound
Immune system
Adenosine Triphosphate
PORPHYROMONAS-GINGIVALIS
NLR Family
Pyrin Domain-Containing 3 Protein
lcsh:Pathology
Humans
Secretion
Caspase
Cells
Cultured
health care economics and organizations
biology
Symporters
Macrophages
Pyroptosis
DEATH
P2X(7) RECEPTOR
EPITHELIAL-CELLS
Cell Biology
Flow Cytometry
Neisseria gonorrhoeae
Cell biology
INTRACELLULAR BACTERIA
030104 developmental biology
chemistry
Caspases
biology.protein
Adenosine triphosphate
Intracellular
HUMAN PHAGOCYTES
lcsh:RB1-214
Research Article
Zdroj: Mediators of Inflammation
Mediators of Inflammation, Vol 2016 (2016)
ISSN: 0962-9351
DOI: 10.1155/2016/1258504
Popis: Neisseria gonorrhoeae(Ngo) has developed multiple immune evasion mechanisms involving the innate and adaptive immune responses. Recent findings have reported that Ngo reduces the IL-1βsecretion of infected human monocyte-derived macrophages (MDM). Here, we investigate the role of adenosine triphosphate (ATP) in production and release of IL-1βin Ngo-infected MDM. We found that the exposure of Ngo-infected MDM to ATP increases IL-1βlevels about ten times compared with unexposed Ngo-infected MDM (P<0.01). However, we did not observe any changes in inflammasome transcriptional activation of speck-like protein containing a caspase recruitment domain (CARD) (ASC,P>0.05) and caspase-1 (CASP1,P>0.05). In addition, ATP was not able to modify caspase-1 activity in Ngo-infected MDM but was able to increase pyroptosis (P>0.01). Notably ATP treatment defined an increase of positive staining for IL-1βwith a distinctive intracellular pattern of distribution. Collectively, these data demonstrate that ATP induces IL-1βsecretion by a mechanism not related to the NLRP3/ASC/caspase-1 axis and likely is acting at the level of vesicle trafficking or pore formation.
Databáze: OpenAIRE
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