Human bladder carcinoma cell lines as indicators of oncogenic change relevant to urothelial neoplastic progression
| Autor: | J. M. Swart, William V. Kastrinakis, Summerhayes Ic, A. F. Little, John A. Libertino, K. M. Rieger, James M. Fitzgerald, D. T. Hess |
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| Rok vydání: | 1995 |
| Předmět: |
Cancer Research
Tumor suppressor gene Molecular Sequence Data Gene Expression medicine.disease_cause Retinoblastoma Protein Proto-Oncogene Proteins medicine Carcinoma Tumor Cells Cultured Humans Genes Tumor Suppressor Nuclear protein Urinary bladder biology Base Sequence Retinoblastoma protein Nuclear Proteins Single-strand conformation polymorphism Proto-Oncogene Proteins c-mdm2 medicine.disease Cadherins Immunohistochemistry medicine.anatomical_structure Oncology Urinary Bladder Neoplasms Cell culture Mutation Cancer research biology.protein Disease Progression Tumor Suppressor Protein p53 Carcinogenesis Research Article |
| Zdroj: | British Journal of Cancer |
| ISSN: | 0007-0920 |
| Popis: | Analysis of human tumour-derived cell lines has previously resulted in the identification of novel transformation-related elements and provided a useful tool for functional studies of different genes. To establish the utility of such cell lines as indicators of change relevant to urothelial cancer, we have characterised the expression of five genes (p53, MDM2, Rb, E-cadherin, APC) within a panel of human bladder carcinoma cell lines. Using single-strand conformation polymorphism (SSCP) and direct sequencing, p53 mutations were identified in 7/15 (47%) cell lines reflecting events reported in bladder tumours. Immunohistochemical analysis of p53 in cultured cells and in paraffin-embedded sections of xenografts from the cell line panel revealed discordant results. An absence of p53 nuclear staining was associated with an exon 5 mutation in EJ and with multiple p53 mutations found in J82. Two cell lines positive for p53 staining in the absence of detectable mutation displayed overexpression of MDM2 (PSI, HT1197) in Western blot analysis. Loss or aberrant Rb expression was recorded in 5/15 (TCCSUP, SCaBER, 5637, HT1376, J82) cell lines. Absence of E-cadherin was recorded in 5/15 cell lines (TCCSUP, EJ, KK47, UM-UC-3, J82) with loss of alpha-catenin in immunoprecipitated E-cadherin complexes of CUBIII. Western blot analysis of APC revealed a truncated protein in 1/15 (CUBIII) cell lines. The characterisation of oncogenic events within this panel of human bladder carcinoma cell lines establishes a representation of change observed in bladder tumours and better defines the genotypic background in these experimental human cell models of neoplastic progression. Images Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Figure 6 |
| Databáze: | OpenAIRE |
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