Biomimetic proteoglycans diffuse throughout articular cartilage and localize within the pericellular matrix
Autor: | Michele Marcolongo, Nicholas Bertha, Maria Lefchak, Brandon Shallop, Mary K. Mulcahey, Evan R. Phillips, Katsiaryna Prudnikova, Joseph Sincavage, Brett D. Haislup |
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Rok vydání: | 2019 |
Předmět: |
Cartilage
Articular Materials science 0206 medical engineering Biomedical Engineering Articular cartilage 02 engineering and technology Matrix (biology) Biomaterials Biomimetic Materials medicine Animals Humans Diffusion (business) Molecular diffusion biology Cartilage Metals and Alloys 021001 nanoscience & nanotechnology 020601 biomedical engineering Fick's laws of diffusion Extracellular Matrix Concentration dependent medicine.anatomical_structure Proteoglycan Ceramics and Composites Biophysics biology.protein Cattle Proteoglycans 0210 nano-technology |
Zdroj: | Journal of Biomedical Materials Research Part A. 107:1977-1987 |
ISSN: | 1552-4965 1549-3296 |
DOI: | 10.1002/jbm.a.36710 |
Popis: | Biomimetic proteoglycan (BPG) diffusion into articular cartilage has the potential to restore the lost proteoglycan content in osteoarthritic cartilage given these molecules mimic the structure and properties of natural proteoglycans. We examined the diffusion characteristics of BPGs through cartilage with the use of a custom-made in vitro cartilage diffusion model in both normal bovine and human osteoarthritic cartilage explants. BPGs were introduced into the cartilage through essentially one-dimensional diffusion using osteochondral plugs. The molecular diffusion was shown to be size and concentration dependent. Diffusion profiles were found over different diffusion time intervals and the profiles were fit to a nonlinear Fickian diffusion model. Steady state 011012-7diffusion coefficients for BPGs were found to be 4.01 and 3.53 μm2 /s for 180 and 1600 kDa BPGs, respectfully, and these values are similar to other large molecule diffusion in cartilage. In both bovine and osteoarthritic human cartilage, BPGs were found localized around the chondrocytes. BPG localization was examined by labeling collagen type VI and soaking 5 μm thick sections of cartilage with BPG solutions demonstrating that the BPGs diffused into the cartilage and preferentially localized alongside collagen type VI in the pericellular matrix. |
Databáze: | OpenAIRE |
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