Analysis of Transforming Growth Factor β and Other Cytokines in Autoimmune Exocrinopathy (Sjögren's Syndrome)
| Autor: | Thomas B. Aufdemorte, Howard Dang, Norman Talal, Noriyoshi Ogawa, Howard S Mcguff, Konstantinos Lazaridis |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty medicine.medical_treatment Molecular Sequence Data Immunology Enzyme-Linked Immunosorbent Assay Salivary Gland Diseases Biology Polymerase Chain Reaction Lesion Transforming Growth Factor beta Virology medicine Humans RNA Messenger Cells Cultured Aged Base Sequence Salivary gland Interleukin Cell Biology Transforming growth factor beta Middle Aged Immunohistochemistry Sjogren's Syndrome medicine.anatomical_structure Cytokine biology.protein Cytokines Female medicine.symptom Immunostaining Transforming growth factor |
| Zdroj: | Journal of Interferon & Cytokine Research. 15:759-767 |
| ISSN: | 1557-7465 1079-9907 |
| DOI: | 10.1089/jir.1995.15.759 |
| Popis: | Cytokines play a major role in tissue destruction caused by autoimmune dysregulation. In Sjögren's syndrome (SS) patients, salivary glands are the target organs for autoimmune tissue damage. In the present study, reverse transcriptase-polymerase chain reaction (RT-PCR) was used to look for cytokine mRNA expressed in SS salivary glands. Focus score was used to determine the severity of the lesions. Cytokine production in supernatants of the salivary gland cell culture was measured by enzyme-linked immunosorbent assay (ELISA). Immunohistochemical staining was used to identify the local presence of transforming growth factor beta (TGF-beta). Interleukin (IL)-2, IL-6, and IL-10 mRNA were expressed in moderate to severe SS salivary gland lesions. TGF-beta mRNA was constitutively expressed in normal and SS salivary glands. In SS salivary gland cell cultures, IL-6 and IL-10 proteins were produced. TGF-beta production was reduced in high focus score SS glands. Normal and minimally involved SS salivary gland ductal epithelium and acinar cells were found to produce TGF-beta by immunostaining. In conclusion, an excess production of IL-2, IL-6, and IL-10 and a reduced production of the immunosuppressive cytokine, TGF-beta, may be responsible for the progression of the salivary gland lesion in SS. Specific immunotherapy can now be designed based on mechanisms to correct this cytokine imbalance and benefit patients with autoimmune diseases, such as SS. |
| Databáze: | OpenAIRE |
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