Involvement of Hypoxia-Inducible Factor 1-α in Experimental Testicular Ischemia and Reperfusion: Effects of Polydeoxyribonucleotide and Selenium

Autor:	Pietro Antonuccio, Giovanni Pallio, Herbert Ryan Marini, Natasha Irrera, Carmelo Romeo, Domenico Puzzolo, Jose Freni, Giuseppe Santoro, Igor Pirrotta, Francesco Squadrito, Letteria Minutoli, Antonio Micali
Jazyk:	angličtina
Rok vydání:	2022
Předmět:	Male testis ischemia-reperfusion rat selenium polydeoxyribonucleotide hypoxia-inducible factor 1-α phosphorylated extracellular signal-regulated kinases 1/2 germinal epithelium Leydig cells NF-E2-Related Factor 2 Organic Chemistry General Medicine Hypoxia-Inducible Factor 1 alpha Subunit Catalysis Computer Science Applications Rats Inorganic Chemistry Rats Sprague-Dawley Selenium Polydeoxyribonucleotides Semen Ischemia Reperfusion Injury Testis Reperfusion Animals Testosterone Physical and Theoretical Chemistry Molecular Biology Spectroscopy
Zdroj:	International Journal of Molecular Sciences; Volume 23; Issue 21; Pages: 13144
ISSN:	1422-0067
DOI:	10.3390/ijms232113144
Popis:	Polydeoxyribonucleotide (PDRN) is an agonist of the A2A adenosine receptor derived from salmon trout sperm. Selenium (Se) is a trace element normally present in the diet. We aimed to investigate the long-term role of PDRN and Se, alone or in association, after ischemia-reperfusion (I/R) in rats. The animals underwent 1 h testicular ischemia followed by 30 days of reperfusion or a sham I/R and were treated with PDRN or Se alone or in association for 30 days. I/R significantly increased hypoxia-inducible factor 1-α (HIF-1α) in Leydig cells, malondialdehyde (MDA), phosphorylated extracellular signal-regulated kinases 1/2 (pErk 1/2), and apoptosis decreased testis weight, glutathione (GSH), testosterone, nuclear factor erythroid 2-related factor 2 (Nrf2), induced testicular structural changes, and eliminated HIF-1α spermatozoa positivity. The treatment with either PDRN or Se significantly decreased MDA, apoptosis, and HIF-1α positivity of Leydig cells, increased testis weight, GSH, testosterone, and Nrf2, and improved the structural organization of the testes. PDRN and Se association showed a higher protective effect on all biochemical, structural, and immunohistochemical parameters. Our data suggest that HIF-1α could play important roles in late testis I/R and that this transcriptional factor could be modulated by PDRN and Se association, which, together with surgery, could be considered a tool to improve varicocele-induced damages.
Databáze:	OpenAIRE
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