Glucocorticoids hamper theex vivomaturation of lung dendritic cells from their low autofluorescent precursors in the human bronchoalveolar lavage: decreases in allostimulatory capacity and expression of CD80 and CD86
| Autor: | Henk C. Hoogsteden, J. M. W. Van Haarst, Hemmo A. Drexhage, H J de Wit, G. T. Verhoeven, Peter J. Simons |
|---|---|
| Přispěvatelé: | Pulmonary Medicine, Immunology |
| Rok vydání: | 2000 |
| Předmět: |
Adult
Isoantigens Adolescent T-Lymphocytes Immunology chemical and pharmacologic phenomena In Vitro Techniques Biology Dexamethasone Fluorescence Antigens CD1 Immune system Antigens CD medicine Humans Immunology and Allergy Glucocorticoids Lung CD86 Membrane Glycoproteins medicine.diagnostic_test Cell adhesion molecule Stem Cells Cell Differentiation hemic and immune systems Dendritic Cells Dendritic cell Middle Aged Mixed lymphocyte reaction Bronchoalveolar lavage B7-1 Antigen Lung Immunology/Disease B7-2 Antigen Lymphocyte Culture Test Mixed Bronchoalveolar Lavage Fluid CD80 Ex vivo |
| Zdroj: | Clinical & Experimental Immunology, 122, 232-240. Wiley-Blackwell Publishing Ltd |
| ISSN: | 1365-2249 0009-9104 |
| DOI: | 10.1046/j.1365-2249.2000.01354.x |
| Popis: | SUMMARYDendritic cells (DCs) were prepared from human bronchoalveolar lavage (BAL) cells. We previously reported that, in particular, the CD1a fraction of the low autofluorescent (LAF) cells contains the precursors for DCs: after overnight culture, 40% of the LAF cells change into functionally and phenotypically prototypic dendritic/veiled cells. There are, as yet, no data on the modulatory effects of glucocorticoids (GC) on the maturation and function of such DCs isolated from the human lung. Functional tests (allogeneic mixed lymphocyte reaction: allo-MLR) were therefore performed with CD1a+ LAF cells at different stimulator-to-T-cell ratios and after preincubation with different dexamethasone (DEX) concentrations. DEX caused suppression of the T-cell stimulatory capacity of CD1a+ LAF cells, which was dose-dependent, and more evident at the higher stimulator-to-T-cell ratios. Here, we also show that CD80 and CD86 are normally expressed at low levels on CD1a+ LAF cell-derived DCs compared to other DC populations. This low-level expression of costimulatory molecules is discussed here in relation to the previously reported low-level expression of CD80 (and CD86) on lung DCs in experimental animals. This appears to play a role in a predominant Th2 cell stimulating potential of DC from the lung environment. DEX exposure of CD1a+ LAF cells prevented the upregulation of even this low-level expression of CD80 and CD86. The veiled/dendritic morphology and the expression of other relevant cell surface markers and adhesion molecules was not affected by DEX exposure. It is concluded that DEX hampers the maturation of CD1a+ LAF cells into active lung DCs. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|