Wakayama Symposium: Modulation of Wound Healing Response in the Corneal Stroma by Osteopontin and Tenascin-C
| Autor: | Yuka Okada, Ai Kitano, Takayoshi Sumioka, Shizuya Saika, Kumi Shirai, Hideaki Kokado, Osamu Yamanaka, Takeshi Miyamoto |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Pathology
medicine.medical_specialty Stromal cell Corneal Stroma Extracellular matrix Neovascularization Fibrosis Cornea medicine Animals Humans Osteopontin Eye Proteins Wound Healing biology Tenascin C Tenascin medicine.disease eye diseases Ophthalmology medicine.anatomical_structure biology.protein Cancer research sense organs medicine.symptom Wound healing Integrin alpha Chains |
| Zdroj: | The Ocular Surface. 11:12-15 |
| ISSN: | 1542-0124 |
| Popis: | The extracellular matrix components osteopontin and tenascin-C are ligands of α9 integrin, and both play roles in corneal wound fibrosis and neovascularization. It has been shown that loss of osteopontin impairs closure of incisional wounds in the mouse cornea. Detailed analyses suggest that the loss of osteopontin reduces macrophage invasion and myofibroblast differentiation in the healing stroma in association with suppression of fibrogenic gene expression in response to injury. Cultured ocular fibroblasts derived from knockout mice showed an impairment of activation of p38 MAPK and Smad3 upon exposure to transforming growth factor β1. The loss of tenascin-C delays stromal healing in association with suppression of fibrogenic gene expression and macrophage invasion. With regard to neovascularization, the loss of either osteopontin or tenascin-C suppressed the growth of new blood vessels from the limbal region toward the central cornea in response to corneal cauterization in mice. Gene expression analysis further showed that lack of osteopontin or tenascin-C resulted in inhibition of angiogenic and proinflammatory gene expression. In conclusion, osteopontin or tenascin-C, α9 integrin ligands, play an important role in stromal healing (or fibrosis) and neovascularization in mouse cornea. |
| Databáze: | OpenAIRE |
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