Sickle Cell Anemia Patients Display an Intricate Cellular and Serum Biomarker Network Highlighted by TCD4+CD69+ Lymphocytes, IL-17/MIP-1β, IL-12/VEGF, and IL-10/IP-10 Axis
| Autor: | Alicia Patrine C. dos Santos, Andréa Monteiro Tarragô, Alexander Leonardo S. Júnior, Olindo Assis Martins-Filho, Nadja Pinto Garcia, Emerson Garcia de Almeida, Geyse Adriana S. Soares, Adriana Malheiro, Rejane Nina Martins, Flávia do Carmo Leão Pontes, Thainá Cristina Cardoso Costa, Erich Vinicius De Paula, Allyson Guimarães Costa |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Adult
Male Chemokine Reticulocytes Article Subject medicine.medical_treatment Immunology Anemia Sickle Cell Adaptive Immunity Proinflammatory cytokine 03 medical and health sciences Young Adult 0302 clinical medicine Immunophenotyping Immune system Th2 Cells medicine Immunology and Allergy Humans 030304 developmental biology 0303 health sciences biology business.industry General Medicine RC581-607 Acquired immune system Healthy Volunteers Interleukin 10 Cytokine 030220 oncology & carcinogenesis Case-Control Studies biology.protein Cytokines Female Interleukin 17 Immunologic diseases. Allergy business Biomarkers Research Article |
| Zdroj: | Journal of Immunology Research Journal of Immunology Research, Vol 2020 (2020) |
| ISSN: | 2314-7156 2314-8861 |
| Popis: | Background. Sickle cell anemia (SCA) is associated with a chronic proinflammatory state characterized by elevated leukocyte count, mortality from severe recurrent infections, and subsequent vasoocclusive complications with leukocyte adhesion to the endothelium and increased plasma levels of inflammatory cytokines. The immune system has a close connection with morbidity in SCA, but further studies are needed to uncover the involvement of innate and adaptive immunities in modulating the SCA physiopathology. We performed measurements of the frequency of innate and adaptive immunity cells, cytokines, chemokines, and growth factors and immunophenotyping of Toll-like receptor and adhesion molecule expression in the blood of SCA patients and healthy donors to evaluate the different profiles of these biomarkers, the relationship among them, and their correlation to laboratory records and death risk. Material and Methods. Immunophenotyping of cells, Toll-like receptors, and adhesion molecules were performed from peripheral blood samples of SCA patients and healthy donors by flow cytometry and cytokine/chemokine/growth factor measurement by the Luminex technique performed from the serum of the same subjects. Results. Cells of adaptive immunity such as IL-12, IL-17, and IL-10 cytokines; IL-8, IP-10, MIP-1α, MIP-1β, and RANTES chemokines; and VEGF, FGF-basic, and GM-CSF growth factors were higher in SCA patients than healthy donors regardless of any laboratorial and clinical condition. However, high death risk appears to have relevant biomarkers. Conclusion. In the SCA pathophysiology at steady state, there is a broad immunological biomarker crosstalk highlighted by TCD4+CD69+ lymphocytes, IL-12 and IL-17 inflammatory and IL-10 regulatory cytokines, MIP-1α, MIP-1β, and IP-10 chemokines, and VEGF growth factor. High expression of TLR2 in monocytes and VLA-4 in TCD8+ lymphocytes and high levels of MIP-1β and RANTES appear to be relevant in high death risk conditions. The high reticulocytosis and high death risk conditions present common correlations, and there seems to be a balance by the Th2 profile. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text