Design, synthesis and biological evaluation of Tozadenant analogues as adenosine A2A receptor ligands
| Autor: | Dirk Bier, Erika Wabbals, Marcus H. Holschbach, Franziska Wedekind, Marcel Skraban, Annette Schulze, Dana R. Renk, Felix Neumaier, Bernd Neumaier |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Pharmacology
0303 health sciences 010405 organic chemistry Chemistry Stereochemistry Organic Chemistry Tozadenant Antagonist Adenosine A2A receptor General Medicine 01 natural sciences Adenosine 0104 chemical sciences 03 medical and health sciences Adenosine A1 receptor Drug Discovery medicine ddc:610 Selectivity Receptor 030304 developmental biology medicine.drug Biological evaluation |
| Zdroj: | European journal of medicinal chemistry 214, 113214 (2021). doi:10.1016/j.ejmech.2021.113214 |
| DOI: | 10.1016/j.ejmech.2021.113214 |
| Popis: | With the aim to obtain potent adenosine A2A receptor (A2AR) ligands, a series of eighteen derivatives of 4-hydroxy-N-(4-methoxy-7-morpholin-4-yl-1,3-benzo[d]thiazol-2-yl)-4-methylpiperidine-1-carboxamide (SYN-115, Tozadenant) were designed and synthesized. The target compounds were obtained by a chemical building block principle that involved reaction of the appropriate aminobenzothiazole phenyl carbamates with either commercially available or readily synthesized functionalized piperidines. Their affinity and subtype selectivity with regard to human adenosine A1-and A2A receptors were determined using radioligand binding assays. Ki values for human A2AR ranged from 2.4 to 38 nM, with more than 120-fold selectivity over A1 receptors for all evaluated compounds except 13k which had a Ki of 361 nM and 18-fold selectivity. The most potent fluorine-containing derivatives 13e, 13g and 13l exhibited Ki values of 4.9 nM, 3.6 nM and 2.8 nM for the human A2AR. Interestingly, the corresponding values for rat A2AR were found to be four to five times higher. Their binding to A2AR was further confirmed by radiolabeling with 18F and in vitro autoradiography in rat brain slices, which showed almost exclusive striatal binding and complete displacement by the A2AR antagonist ZM 241385. We conclude that these compounds represent potential candidates for the visualization of the A2A receptor and open pathways to novel therapeutic treatments of neurodegenerative disorders or cancer. |
| Databáze: | OpenAIRE |
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