Induction of Myogenic Differentiation Improves Chemosensitivity of Chemoresistant Cells in Soft-Tissue Sarcoma Cell Lines
| Autor: | Abraham Hakim, Richard D Lackman, Spencer A. Brown, Luca D'Agostino, Richard Sensenig, Igor Kuzin, Zeus A. Antonello, Lucy E Dawson |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Vincristine animal structures Article Subject medicine.medical_treatment 03 medical and health sciences 0302 clinical medicine medicine Radiology Nuclear Medicine and imaging Doxorubicin Noggin Rhabdomyosarcoma RC254-282 Myogenin Chemotherapy business.industry Soft tissue sarcoma Neoplasms. Tumors. Oncology. Including cancer and carcinogens medicine.disease 030104 developmental biology Oncology Cell culture 030220 oncology & carcinogenesis embryonic structures Cancer research business Research Article medicine.drug |
| Zdroj: | Sarcoma Sarcoma, Vol 2020 (2020) |
| ISSN: | 1357-714X |
| DOI: | 10.1155/2020/8647981 |
| Popis: | Rhabdomyosarcoma (RMS) and rhabdoid tumors (RT) are rare soft-tissue malignancies with the highest incidence in infants, children, and adolescents. Advanced, recurrent, and/or metastatic RMS and RT exhibit poor response to treatment. One of the main mechanisms behind resistance to treatment is believed to be intratumoral heterogeneity. In this study, we investigated the myogenic determination factor 1 (MYOD1) and Noggin (NOG) markers in an embryonal RMS (ERMS) cell line and an RT cell line and the differential response of the MYOD1 and NOG expressing subpopulations to chemotherapy. Importantly, we found that these markers together identify a subpopulation of cells (MYOD1+ NOG+ cells) with primary resistance to Vincristine and Doxorubicin, two commonly used chemotherapies for ERMS and RT. The chemoresistant MYOD1+ NOG+ cells express markers of undifferentiated cells such as myogenin and ID1. Combination of Vincristine with TPA/GSK126, a drug combination shown to induce differentiation of RMS cell lines, is able to partially overcome MYOD1/NOG cells chemoresistance. |
| Databáze: | OpenAIRE |
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