False-positive Rifampin Resistant Results with Xpert MTB/RIF Version 4 Assay in clinical samples with a low bacterial load

Autor:	Erin Byrt, Marie-Marcelle Mabou, Vanessa Rouzier, Oksana Ocheretina, Yves-Mary Merveille, Gertrude Royal-Mardi, Daniel W. Fitzgerald, Jean W. Pape
Jazyk:	angličtina
Rok vydání:	2016
Předmět:	0301 basic medicine Microbiology (medical) Silent mutation Tuberculosis 030106 microbiology Microbial Sensitivity Tests Bioinformatics Real-Time Polymerase Chain Reaction Article 03 medical and health sciences mental disorders Tuberculosis Multidrug-Resistant medicine polycyclic compounds Humans Tuberculosis Pulmonary business.industry Sputum Reproducibility of Results General Medicine Mycobacterium tuberculosis biochemical phenomena metabolism and nutrition rpoB medicine.disease Molecular diagnostics bacterial infections and mycoses Virology Bacterial Load Infectious Diseases Mutation Rifampin business True positive rate psychological phenomena and processes
Popis:	We report investigation of 22 TB cases with positive Xpert MTB/RIF result for resistance to Rifampin and "Very Low" MTB detection level. Twelve cases were false positive without rpoB mutations, 2 were false-positives with a silent mutation in rpoB codon T508, and only 10 were true positives.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ed7942c14cfe6fad5eb7e25ae3ab5327 https://europepmc.org/articles/PMC4841693/ Zobrazit plný text záznamu