Insulin Pulse Characteristics and Insulin Action in Non-diabetic Humans
| Autor: | Adrian Vella, Giuseppe De Nicolao, John G. Jones, Marcello C. Laurenti, James C. Andrews, Robert A. Rizza, Chiara Dalla Man, Aleksey V. Matveyenko, Cristina Barosa, Claudio Cobelli, Kent R. Bailey, Ron T. Varghese |
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| Rok vydání: | 2021 |
| Předmět: |
Adult
Blood Glucose Male glucose disappearance medicine.medical_specialty Pulsatile insulin Endocrinology Diabetes and Metabolism medicine.medical_treatment Clinical Biochemistry Type 2 diabetes Biochemistry Endocrinology Insulin resistance Internal medicine Insulin Secretion C-peptide kinetics endogenous glucose production gluconeogenesis insulin action insulin pulses Humans Insulin Medicine Clinical Research Articles C-Peptide biology Pulse (signal processing) business.industry Biochemistry (medical) Fasting Middle Aged medicine.disease Insulin receptor Glucose Liver Basal (medicine) Gluconeogenesis Glucose Clamp Technique biology.protein Female Insulin Resistance business |
| Zdroj: | J Clin Endocrinol Metab |
| ISSN: | 1945-7197 0021-972X |
| Popis: | Objective Pulsatile insulin secretion is impaired in diseases such as type 2 diabetes that are characterized by insulin resistance. This has led to the suggestion that changes in insulin pulsatility directly impair insulin signaling. We sought to examine the effects of pulse characteristics on insulin action in humans, hypothesizing that a decrease in pulse amplitude or frequency is associated with impaired hepatic insulin action. Methods We studied 29 nondiabetic subjects on two occasions. On 1 occasion, hepatic and peripheral insulin action was measured using a euglycemic clamp. The deuterated water method was used to estimate the contribution of gluconeogenesis to endogenous glucose production. On a separate study day, we utilized nonparametric stochastic deconvolution of frequently sampled peripheral C-peptide concentrations during fasting to reconstruct portal insulin secretion. In addition to measuring basal and pulsatile insulin secretion, we used approximate entropy to measure orderliness and Fourier transform to measure the average, and the dispersion of, insulin pulse frequencies. Results In univariate analysis, basal insulin secretion (R2 = 0.16) and insulin pulse amplitude (R2 = 0.09) correlated weakly with insulin-induced suppression of gluconeogenesis. However, after adjustment for age, sex, and weight, these associations were no longer significant. The other pulse characteristics also did not correlate with the ability of insulin to suppress endogenous glucose production (and gluconeogenesis) or to stimulate glucose disappearance. Conclusions Overall, our data demonstrate that insulin pulse characteristics, considered independently of other factors, do not correlate with measures of hepatic and peripheral insulin sensitivity in nondiabetic humans. |
| Databáze: | OpenAIRE |
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