Application of BAX system, Tecra Unique Salmonella test, and a conventional culture method for the detection of Salmonella in ready-to-eat and raw foods
| Autor: | K.K. Kwok, K.M. Kam, P.-Y. Cheung |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Serotype
Salmonella typhimurium Salmonella Meat Salmonella serotype typhi Sus scrofa Ready to eat Biology medicine.disease_cause Applied Microbiology and Biotechnology Poultry Microbiology medicine Animals False Negative Reactions Bacteriological Techniques Salmonella serotype Typhimurium Reproducibility of Results General Medicine Bacterial Typing Techniques Sensitivity test Salmonella enteritidis SALMONELLA SEROTYPE ENTERITIDIS Food Microbiology Reagent Kits Diagnostic Biotechnology |
| Zdroj: | Journal of applied microbiology. 103(1) |
| ISSN: | 1364-5072 |
| Popis: | Aims: To compare the BAX system, the Tecra UniqueTM Salmonella test, and a conventional culture method for the detection of Salmonella in various foods. Methods and Results: Ready-to-eat and raw foods were inoculated with Salmonella serotype Typhimurium, Salmonella serotype Enteritidis, Salmonella serotype Typhi, or Salmonella serotype Derby. Incubated pre-enrichment cultures were examined using the BAX system, the Tecra UniqueTM Salmonella test, and a conventional culture method. Salmonella could be detected in all ready-to-eat food samples inoculated with S. Typhimurium, S. Enteritidis, or S. Derby, with any of the three test methods. However, false negatives were obtained with the Tecra test and the culture method when samples with higher background flora were inoculated with S. Typhi. Sensitivity test results suggested the two rapid tests performed as well as the culture method in the detection of 101 CFU of S. Typhimurium in 25-g cooked or raw food. Conclusions: The BAX system and the Tecra UniqueTM Salmonella test demonstrated results comparable with those of the culture method in the detection of Salmonella serotypes used except S. Typhi. Significance and Impact of the Study: This is the first evaluation of the BAX system, the Tecra UniqueTM Salmonella test, and a culture method in the detection of Salmonella in a variety of western and oriental foods. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text