Enzyme immunoassay for detection of human immunodeficiency virus-specific immunoglobulin A antibodies
| Autor: | Edward M. Connor, Richard Stephens, Bart Holland, James M. Oleske, George D. McSherry, Paul Palumbo, Thomas N. Denny, Zhifu Wang |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Adult
Male Microbiology (medical) Immunoglobulin A Adolescent Blotting Western HIV Infections HIV Antibodies Immunoglobulin E Sensitivity and Specificity Virus Immunoenzyme Techniques Acquired immunodeficiency syndrome (AIDS) Western blot Antibody Specificity Predictive Value of Tests medicine Humans Child Finland Aged biology medicine.diagnostic_test business.industry Infant virus diseases Middle Aged medicine.disease Virology Child Preschool Immunoassay Immunology HIV-1 biology.protein Female Viral disease Antibody business Research Article |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.31.3.681-684.1993 |
| Popis: | Early diagnosis of human immunodeficiency virus (HIV) infection may be difficult in adults with acute or recent HIV infection and in infants with perinatally acquired HIV. Detection of HIV-specific immunoglobulin A (IgA) antibodies in infant serum by Western blot (immunoblot) has been suggested as a reliable method to identify HIV-infected infants, especially those over the age of 6 months, and as an adjunct to diagnosis of acute HIV infection in adults. We developed a simple enzyme immunoassay for detection of HIV-specific IgA, using standard commercially available reagents. Enzyme immunoassay was comparable to Western blot for detection of HIV-specific IgA in sera from adults (n = 216), older children (n = 49), and infants born to HIV-infected mothers (n = 65). Specificity was 100% and sensitivity ranged from 80 to 92%. IgA-enzyme immunoassay is a simple, highly sensitive method for detection of HIV-specific IgA antibodies and is easily adapted to the standard clinical laboratory. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|